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• FAM-11-dUTP, 6-isomer

FAM-11-dUTP, 6-isomer

6-FAM-11-2’-deoxyuridine-5’-triphosphate, trilithium salt, is a common agent for non-radioactive DNA labeling.

FAM (fluorescein) is a popular green-fluorescent dye with an emission maximum at 513 nm. This derivative is a pure FAM 6-isomer.

6-FAM-11-dUTP is a modified triphosphate that can be incorporated into DNA instead of dTTP by various enzymes, including Klenow fragment, Taq-Polymerase, DNA-Polymerase I, Phi 29 Polymerase, reverse transcriptase or terminal transferase.

In contrast to triphosphates with a dye directly linked to aminoallyl-dUTP, this triphosphate contains a linker of 11 atoms between the fluorophore and the nitrogenous base. This linker length prevents FAM-label from potential static quenching and increases the efficiency of nucleotide incorporation during DNA synthesis.

6-FAM-11-dUTP can be used to produce a labeled product during PCR and cDNA synthesis and for labeled DNA-probes synthesis by random primed labeling and Nick-translation. Synthesized fluorescence-labeled DNA probes can be used to detect specific sequences by Southern blot, Northern blot, in situ hybridization, or by microarray analysis.

6-FAM-11-2′-deoxyuridine-5′-triphosphate，三锂盐，是非放射性 DNA 标记的常用试剂​​。

FAM（荧光素）是一种流行的绿色荧光染料，最大发射波长为 513 nm。该衍生物是纯 FAM 6 异构体。

6-FAM-11-dUTP 是一种修饰的三磷酸盐，可以通过各种酶（包括 Klenow 片段、Taq-聚合酶、DNA-聚合酶 I、Phi 29 聚合酶、逆转录酶或末端转移酶）掺入 DNA 中，而不是 dTTP。

与染料直接连接到氨基烯丙基-dUTP 的三磷酸盐相比，这种三磷酸盐在荧光团和含氮碱基之间含有 11 个原子的接头。这种接头长度可防止 FAM 标记潜在的静态猝灭，并提高 DNA 合成过程中核苷酸掺入的效率。

6-FAM-11-dUTP 可用于在 PCR 和 cDNA 合成过程中产生标记产物，以及通过随机引物标记和尼克翻译合成标记的 DNA 探针。合成的荧光标记 DNA 探针可用于通过Southern印迹、Northern印迹、原位杂交或微阵列分析检测特定序列。