FAM-11-dUTP, 6-isomer

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  • FAM-11-dUTP, 6-isomer

FAM-11-dUTP, 6-isomer

标签:FAM (Fluorescein), PCR and qPCR, Triphosphates
货号 规格 价格 货期
2077-50nmol 50 nmol 190.00$ in stock
2077-250nmol 250 nmol 750.00$ in stock
2077-500nmol 500 nmol 1200.00$ 7 days
2077-1umol 1 umol please inquire in stock

6-FAM-11-2’-deoxyuridine-5’-triphosphate, trilithium salt, is a common agent for non-radioactive DNA labeling.

FAM (fluorescein) is a popular green-fluorescent dye with an emission maximum at 513 nm. This derivative is a pure FAM 6-isomer.

6-FAM-11-dUTP is a modified triphosphate that can be incorporated into DNA instead of dTTP by various enzymes, including Klenow fragment, Taq-Polymerase, DNA-Polymerase I, Phi 29 Polymerase, reverse transcriptase or terminal transferase.

In contrast to triphosphates with a dye directly linked to aminoallyl-dUTP, this triphosphate contains a linker of 11 atoms between the fluorophore and the nitrogenous base. This linker length prevents FAM-label from potential static quenching and increases the efficiency of nucleotide incorporation during DNA synthesis.

6-FAM-11-dUTP can be used to produce a labeled product during PCR and cDNA synthesis and for labeled DNA-probes synthesis by random primed labeling and Nick-translation. Synthesized fluorescence-labeled DNA probes can be used to detect specific sequences by Southern blot, Northern blot, in situ hybridization, or by microarray analysis.

6-FAM-11-2′-deoxyuridine-5′-triphosphate,三锂盐,是非放射性 DNA 标记的常用试剂​​。

FAM(荧光素)是一种流行的绿色荧光染料,最大发射波长为 513 nm。该衍生物是纯 FAM 6 异构体。

6-FAM-11-dUTP 是一种修饰的三磷酸盐,可以通过各种酶(包括 Klenow 片段、Taq-聚合酶、DNA-聚合酶 I、Phi 29 聚合酶、逆转录酶或末端转移酶)掺入 DNA 中,而不是 dTTP。

与染料直接连接到氨基烯丙基-dUTP 的三磷酸盐相比,这种三磷酸盐在荧光团和含氮碱基之间含有 11 个原子的接头。这种接头长度可防止 FAM 标记潜在的静态猝灭,并提高 DNA 合成过程中核苷酸掺入的效率。

6-FAM-11-dUTP 可用于在 PCR 和 cDNA 合成过程中产生标记产物,以及通过随机引物标记和尼克翻译合成标记的 DNA 探针。合成的荧光标记 DNA 探针可用于通过Southern印迹、Northern印迹、原位杂交或微阵列分析检测特定序列。

Absorption and emission spectra of FAM

FAM-11-dUTP, 6-isomer

FAM-11-dUTP, 6-isomer相关产品

Sulfo-Cyanine5 dUTP

Sulfo-Cyanine5 dUTP is a fluorescent labeled triphosphate for the enzymatic modification of DNA with sulfo-Cyanine5 fluorophore. The reagent contains a long linker arm, its incorporation is highly efficient.

Sulfo-Cy5 dUTP 是一种荧光标记的三磷酸盐,用于用 sulfo-Cy5 荧光团对 DNA 进行酶促修饰。 该试剂含有一个长连接臂,它的掺入效率很高。

Cyanine5 NHS ester minimal dye

Cy5 NHS ester minimal dye for protein labeling.

Cy5 NHS 酯最小染料用于蛋白质标记。


Amino-11-dUTP is a triphosphate for the modification of DNA.

Amino-11-dUTP 是一种用于修饰 DNA 的三磷酸盐。

General properties

Appearance: yellow-greenish solid 黄绿色固体
Molecular weight: 1013.49
Molecular formula: C39H39N4Li3O21P3
IUPAC name: ((2R,3S,5R)-5-(5-(3-(6-(3-carboxy-4-(6-hydroxy-3-oxo-3H-xanthen-9-yl)benzamido)hexanamido)prop-1-yn-1-yl)-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-3-hydroxytetrahydrofuran-2-yl)methyl hydrogen triphosphate
Solubility: soluble in water
Quality control: HPLC-MS (95%), testing in enzymatic reaction
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light and excessive freeze-thaw cycles.

储存:收到后 12 个月,在 -20°C 避光保存。 运输:在室温下长达 3 周。 避免长时间暴露在光线下和过度冻融循环。

MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 490
ε, L⋅mol−1⋅cm−1: 80000
Emission maximum, nm: 513
Fluorescence quantum yield: 0.93
CF260: 0.20
CF280: 0.17