FAM-11-dCTP, 6-isomer

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  • FAM-11-dCTP, 6-isomer

FAM-11-dCTP, 6-isomer

标签:FAM (Fluorescein), PCR and qPCR, Triphosphates
货号 规格 价格 货期
2713-50nmol 50 nmol 190.00$ in stock
2713-250nmol 250 nmol 750.00$ in stock
2713-500nmol 500 nmol 1200.00$ in stock
2713-1umol 1 umol please inquire in stock
6-fluorescein (FAM) derivative of deoxycytidine triphosphate (dCTP).

FAM 6-isomer is a common fluorophore used for labeling biomolecules; FAM has a high quantum yield and bright green emission.

FAM-11-dCTP can be used for enzymatic DNA labeling via Nick-translation, 3’-end labeling, random primed labeling, PCR, or cDNA synthesis. FAM-11-dCTP is incorporated into the growing DNA chain by Taq-polymerase, DNA-polymerase I, Phi 29 polymerase, Klenow fragment, reverse transcriptase, or terminal transferase.

The linker length (11 carbon atoms) is optimal for the efficient incorporation of fluorescently-labeled dCTP into the growing DNA chain.

Labeled DNA can be used for FISH experiments, microarray gene profiling, Southern blot, and Northern blot.

脱氧胞苷三磷酸 (dCTP) 的 6-荧光素 (FAM) 衍生物。
FAM 6 异构体是用于标记生物分子的常见荧光团; FAM 具有高量子产率和亮绿色发射。

FAM-11-dCTP 可用于通过尼克翻译、3′ 端标记、随机引物标记、PCR 或 cDNA 合成进行酶促 DNA 标记。 FAM-11-dCTP 通过 Taq 聚合酶、DNA 聚合酶 I、Phi 29 聚合酶、Klenow 片段、逆转录酶或末端转移酶结合到不断增长的 DNA 链中。

接头长度(11 个碳原子)对于将荧光标记的 dCTP 有效掺入正在生长的 DNA 链中是最佳的。

标记的 DNA 可用于 FISH 实验、微阵列基因分析、Southern 印迹和 Northern 印迹。

Absorption and emission spectra of FAM

FAM-11-dCTP, 6-isomer

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dsGreen for Real-Time PCR, 100×

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FAM-11-dCTP, 6-isomer Add this product to your cart and
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General properties

Appearance: yellow/orange solid 黄色/橙色固体
Molecular weight: 1012.5
Molecular formula: C39H40N5Li3O20P3
IUPAC name: ((2R,3S,5R)-5-(4-amino-5-(3-(6-(3-carboxy-4-(6-hydroxy-3-oxo-3H-xanthen-9-yl)benzamido)hexanamido)prop-1-yn-1-yl)-2-oxo-3,4-dihydropyrimidin-1(2H)-yl)-3-hydroxytetrahydrofuran-2-yl)methyl hydrogen triphosphate
Solubility: good in water
Quality control: HPLC-MS (95%), testing in enzymatic reaction
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light and excessive freeze-thaw cycles.

储存:收到后 12 个月,在 -20°C 避光保存。 运输:在室温下长达 3 周。 避免长时间暴露在光线下和过度冻融循环。

MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 490
ε, L⋅mol−1⋅cm−1: 80000
Emission maximum, nm: 513
Fluorescence quantum yield: 0.93
CF260: 0.20
CF280: 0.17

FAM-11-dCTP, 6-isomer