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  • Biotin-11-dUTP


标签:PCR and qPCR, Triphosphates
货号 规格 价格 货期
1553-250nmol 250 nmol in stock
1553-500nmol 500 nmol 190.00$ in stock
1553-1umol 1 umol 290.00$ in stock
1553-5umol 5 umol please inquire 21 days

Biotinylated deoxyuridine triphosphate (dUTP) can be used for DNA labeling using various methods, including Nick-translation, polymerase chain reaction (PCR), random priming, and 3’-terminal non-radioactive labeling. Generated probes can be used in various hybridization experiments such as Southern blot, Northern blot, dot blot, or FISH. Biotin-labeled probes are detected with horseradish peroxidase-conjugated streptavidin or other biomolecule conjugates binding biotin.

«11» in the compound name indicates the length of the linker between dUTP and biotin; this linker increases the efficiency of labeled dUTP incorporation into DNA and interaction of biotin with specific proteins such as avidin/streptavidin or anti-biotin antibodies.

生物素化脱氧尿苷三磷酸 (dUTP) 可用于使用各种方法进行 DNA 标记,包括尼克翻译、聚合酶链式反应 (PCR)、随机引发和 3′-末端非放射性标记。 生成的探针可用于各种杂交实验,例如Southern印迹、Northern印迹、斑点印迹或FISH。 用辣根过氧化物酶偶联的链霉亲和素或其他结合生物素的生物分子偶联物检测生物素标记的探针。

化合物名称中的 «11» 表示 dUTP 和生物素之间的接头长度; 该接头提高了标记的 dUTP 掺入 DNA 的效率以及生物素与特定蛋白质(如抗生物素蛋白/链霉抗生物素蛋白或抗生物素抗体)的相互作用。


Sulfo-Cyanine5 dUTP

Sulfo-Cyanine5 dUTP is a fluorescent labeled triphosphate for the enzymatic modification of DNA with sulfo-Cyanine5 fluorophore. The reagent contains a long linker arm, its incorporation is highly efficient.

Sulfo-Cy5 dUTP 是一种荧光标记的三磷酸盐,用于用 sulfo-Cy5 荧光团对 DNA 进行酶促修饰。 该试剂含有一个长连接臂,它的掺入效率很高。


Amino-11-dUTP is a triphosphate for the modification of DNA.

Amino-11-dUTP 是一种用于修饰 DNA 的三磷酸盐。

FAM-11-dUTP, 6-isomer

FAM-derivative of dUTP with green emission for DNA labeling.

用于 DNA 标记的具有绿色发射的 dUTP 的 FAM 衍生物。

General properties

Appearance: colorless solid 无色固体
Molecular weight: 881.48
Molecular formula: C28H43N6Li3O17P3S
IUPAC name: ((2R,3S,5R)-5-(2,4-dioxo-5-(3-(6-(5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamido)hexanamido)prop-1-yn-1-yl)-3,4-dihydropyrimidin-1(2H)-yl)-3-hydroxytetrahydrofuran-2-yl)methyl hydrogen triphosphate
Solubility: soluble in water
Quality control: HPLC-MS (95%), testing in enzymatic reaction
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid excessive freeze-thaw cycles.

储存:收到后 12 个月,在 -20°C 避光保存。 运输:在室温下长达 3 周。 避免过多的冻融循环。

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Product specifications