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• Amino-11-UTP

Amino-11-UTP

Amino-UTP is uridine triphosphate with an amino group, which is designed for enzymatic incorporation of the amino group into the RNA molecule during in vitro transcription using T7, T3, and SP6 RNA polymerases. The enzymatic reaction results in the RNA molecule with amino groups, which can be conjugated with amine-reactive reagents such as NHS esters of fluorescent dyes or biotin.

The amino group is separated from uridine with a long linker of 11 atoms; it is longer than frequently used C3-allyl linker and prevents the fluorophore from potential static quenching after RNA labeling via the amino group. The efficacy of RNA synthesis using amino-11-UTP is almost the same as that when using non-labeled nucleotide triphosphates.

Incorporation of amino-11-UTP with subsequent labeling via the amino group is used for cRNA synthesis and microarray analysis.

氨基-UTP 是带有氨基的三磷酸尿苷，设计用于在使用 T7、T3 和 SP6 RNA 聚合酶进行体外转录期间将氨基酶促掺入 RNA 分子中。 酶促反应产生带有氨基的 RNA 分子，可与胺反应性试剂（如荧光染料的 NHS 酯或生物素）结合。

氨基通过 11 个原子的长接头与尿苷分离； 它比常用的 C3-烯丙基接头更长，可防止荧光团在通过氨基标记 RNA 后发生潜在的静态猝灭。 使用氨基 11-UTP 合成 RNA 的效果几乎与使用未标记的三磷酸核苷酸相同。

将氨基-11-UTP 与随后通过氨基标记的结合用于 cRNA 合成和微阵列分析。