ROX reference dye for qPCR

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  • ROX reference dye for qPCR

ROX reference dye for qPCR

标签:ROX (Rhodamine X, Rhodamine 101), PCR and qPCR
货号 规格 价格 货期
31110 500 uL 22.00$ in stock
41110 1 mL 30.00$ in stock
51110 25 mL 230.00$ in stock
61110 50mL 300.00$ in stock

This ROX formulation is a passive reference dye for qPCR application. The use of this dye is recommended for the normalization of fluorescence intensity of qPCR reporter dyes, such as dsGreen or TaqMan probes. It also allows to correct for pipeting errors, and instrumental drift such as change of lamp intensity output over time.

The dye contains 25 μM solution of 5-carboxy-ROX (rhodamine-X) reference dye in 10mM Tris-HCl (pH 8.6), 0.1mM EDTA, and 0.01% Tween®-20.

ROX reference dye is compatible with all qPCR instruments with ROX channel. Most instruments require either 500 nM or 50 nM end concentration of ROX.

这种 ROX 配方是用于 qPCR 应用的被动参考染料。 推荐使用这种染料来标准化 qPCR 报告染料的荧光强度,例如 dsGreen 或 TaqMan 探针。 它还允许纠正移液错误和仪器漂移,例如灯强度输出随时间的变化。

该染料含有 25 μM 5-羧基-ROX (rhodamine-X) 参比染料在 10mM Tris-HCl (pH 8.6)、0.1mM EDTA 和 0.01% Tween®-20 中的溶液。

ROX 参考染料与所有带有 ROX 通道的 qPCR 仪器兼容。 大多数仪器需要 500 nM 或 50 nM 的 ROX 末端浓度。

ROX reference dye for qPCR相关产品

Cyanine3 amine

Cyanine3 is an fluorophore with free amino group (amino-dye). It can be conjugated with NHS esters, carboxy groups (after activation), and epoxides.

Cy3 是一种带有游离氨基的荧光团(氨基染料)。 它可以与 NHS 酯、羧基(活化后)和环氧化物结合。

Sulfo-Cyanine7 carboxylic acid

Sulfo-Cy7 carboxylic acid is a non-reactive fluorescent dye which can be used as a NIR (near infrared) marker.

Sulfo-Cy7 羧酸是一种非反应性荧光染料,可用作 NIR(近红外)标记。

Sulfo-Cyanine3 carboxylic acid

Sulfo-Cyanine3 free unactivated monofunctional carboxylic acid, water-soluble dye.

Sulfo-Cy3 不含未活化的单官能羧酸,水溶性染料。

General properties

Appearance: pink solution
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.

储存:收到后 24 个月,在 -20°C 避光保存。 运输:在室温下长达 3 周。 避免长时间暴露在光线下。

MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 570
Emission maximum, nm: 591


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