Biotin-PEG3-Azide

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Biotin-PEG3-Azide

标签:Azides, PEG3 (triethylene glycol)
货号 规格 价格 货期
C3730 10 mg 40.00$ in stock
D3730 25 mg 90.00$ in stock
E3730 50 mg 140.00$ in stock
F3730 100 mg 180.00$ in stock

Biotin azide is a derivative of biotin (vitamin H) covalently bound with azide group. This reagent allows labeling of various alkynylated molecules (such as DNA, oligonucleotides, and proteins) with biotin. Biotin binding to avidin or streptavidin can be used in downstream affinity applications for the isolation of biotinylated molecules or their binding with streptavidin conjugates.

This structure of biotin azide features long hydrophilic linker which separates biotin residue from the target molecule for efficient binding with streptavidin. Its linker (PEG) also enhances aqueous solubility to facilitate conjugation.

The azide can be conjugated with various molecules using our recommended protocol.

生物素叠氮化物是与叠氮基共价结合的生物素(维生素 H)的衍生物。 该试剂允许用生物素标记各种炔基化分子(如 DNA、寡核苷酸和蛋白质)。 与亲和素或链霉亲和素结合的生物素可用于下游亲和应用,以分离生物素化分子或它们与链霉亲和素缀合物的结合。

生物素叠氮化物的这种结构具有长的亲水性接头,可将生物素残基与靶分子分开,从而与链霉亲和素有效结合。 它的接头 (PEG) 还增强了水溶性以促进结合。

使用我们推荐的方案,叠氮化物可以与各种分子结合。

Biotin-PEG3-Azide相关产品

Cyanine3 NHS ester

Amine-reactive Cyanine3 dye NHS ester.

胺反应性 Cy3 染料 NHS 酯。

PEP azide

PEP (phenylethynylpyrene), polyaromatic hydrocarbon fluorescent dye for Click-Chemistry labeling.

PEP(苯乙炔基芘),用于 Click-Chemistry 标记的多环芳烃荧光染料。

Cyanine3.5 azide

Cyanine3.5 azide for biomolecule labeling through Click Chemistry.

通过 Click Chemistry 用于生物分子标记的 Cy3.5 叠氮化物。

General properties

Appearance: colorless solid 无色固体
Molecular weight: 400.50
CAS number: 945633-30-7
Molecular formula: C16H28N6O4S
Solubility: soluble in DMF, DMSO, moderately soluble in water
Quality control: NMR 1H (95%) and 13C, TLC, functional testing
Storage conditions: Storage: 24 months after receival at -20°C. Transportation: at room temperature for up to 3 weeks.

储存:-20°C 收货后 24 个月。 运输:在室温下长达 3 周。

MSDS: Download
Product specifications

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  1. Dettling, S.E.; Ahmadi, M.; Lin, Z.; He, L.; Matthews, M.L. Discovery of Electrophiles and Profiling of Enzyme Cofactors. Current Protocols in Chemical Biology, 2020, 12(4), e86. doi: 10.1002/cpch.86
  2. Wang, Y.; Dattmore, D.A.; Wang, W:; Pohnert, G.; Wolfram, S.; Zhang, J.; Yang, R.; Decker, E.A.; Lee, K.S.S.; Zhang, G. trans, trans-2,4-decadienal, a lipid peroxidation product, induces inflammatory responses via Hsp90- or 14–3-3ζ-dependent mechanisms. Journal of Nutritional Biochemistry, 2020, 76, 108286. doi: 10.1016/j.jnutbio.2019.108286
  3. Shen, Y.; Yang, S.; Hu, X.; Zhang, M.; Ma, X.; Wang, Z.; Hou, Y.; Bai, G. Natural product puerarin activates Akt and ameliorates glucose and lipid metabolism dysfunction in hepatic cells. Journal of Functional Foods, 2019, 55, 296–304. doi: 10.1016/j.jff.2019.02.035
  4. Wang, E.; Hunter, C.P. SID-1 Functions in Multiple Roles To Support Parental RNAi in Caenorhabditis elegans. Genetics, 2017, 207(2), 547–557. doi: 10.1534/genetics.117.300067