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• AmdU (5-azidomethyl-2′-deoxyuridine)

AmdU (5-azidomethyl-2′-deoxyuridine)

Unlike EdU that needs to be further modified with azides in the presence of copper catalyst, AmdU can also react in the absence of copper catalyst using spAAC Click Chemistry with strained cycloalkynes, such as cyclooctynes. This enables detection of nascent DNA in benign, copper free conditions.

叠氮甲基-dU (AmdU) 是一种含有叠氮基的核苷。 核苷的结构类似于胸苷，它通过细胞聚合酶掺入新生DNA中，类似于EdU。

与需要在铜催化剂存在下用叠氮化物进一步改性的 EdU 不同，AmdU 也可以在没有铜催化剂的情况下使用 spAAC Click Chemistry 与应变的环炔烃（例如环辛炔）发生反应。 这使得在良性、无铜条件下检测新生 DNA 成为可能。