lumiprobe -Cyanine3 tetrazine

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Reactive dyes
Tetrazines
Cyanine3 tetrazine

Cyanine3 tetrazine

Cyanine3 Tetrazine Copper free Click chemistry

Cat. #	Quantity	Price
110E0	1 mg	110.00$
210E0	5 mg	290.00$
410E0	25 mg	690.00$
510E0	50 mg	1270.00$
610E0	100 mg	1990.00$

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Items in stock will be shipped on 24.01.2022

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Cyanine3 tetrazine is a fluorescent dye with tetrazine moiety for a conjugation reaction called TCO (trans-cyclooctene) ligation. Actually, tetrazines react not only with trans-cyclooctenes, but also with (methyl)cyclopropenes and strained non-benzo-fused cycloalkynes.

Cyanine3 is a moderately hydrophobic dye, enabling labeling in non-aqueous environment as well as in aqueous media containing some organic co-solvent like acetonitrile or DMSO. For reactions in purely aqueous media, a sulfonated version of this reagent is available.

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Absorption and emission spectra of Cyanine3

Cyanine3 tetrazine

Spectral properties

Excitation/absorption maximum, nm:	555
ε, L⋅mol⁻¹⋅cm⁻¹:	150000
Emission maximum, nm:	570
Fluorescence quantum yield:	0.31
CF₂₆₀:	0.04
CF₂₈₀:	0.09

Bai, M.; Cao, X.; Huang, W.; Chen, F.; Zhao, Y.; Xue, J.; Li, Y.; Cheng, Y.; Zhang, L.; Zhao, Y. Visualizing Newly Synthesized RNA by Bioorthogonal Labeling-primed DNA Amplification. Analytical Chemistry, 2020, 92(12), 8444–8449. doi: 10.1021/acs.analchem.0c01161
Moutsiopoulou, A.; Broyles, D.; Joda, H.; Dikici, E.; Kaur, A.; Kaifer, A.E.; Daunert, S.; Deo, S.K. Bioluminescent Protein-Inhibitor Pair in the Design of a Molecular Aptamer Beacon Biosensing System. Analytical Chemistry, 2020, 92(11), 7393–7398. doi: 10.1021/acs.analchem.0c00518
Chen, F.; Bai, M.; Cao, X.; Zhao, Y.; Xue, J.; Zhao, Y. Click-encoded rolling FISH for visualizing single-cell RNA polyadenylation and structures. Nucleic Acids Research, 2019, 47(22), e145. doi: 10.1093/nar/gkz852

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lumiprobe -Cyanine3 tetrazine

Cyanine3 tetrazine

Absorption and emission spectra of Cyanine3

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Spectral properties