AF488 carboxylic acid

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AF488 carboxylic acid

货号 规格 价格 货期
11890 1 mg 110.00$ in stock
21890 5 mg 290.00$ in stock
41890 25 mg 690.00$ in stock
51890 50 mg 1270.00$ in stock
61890 100 mg please inquire 21 days

AF488 is a bright green-fluorescent dye that is commonly used in microscopy and cell assays because of its photostability. AF488 can be used with DAPI and is well suited to multiplex assay. This high quantum yield dye has fluorescence stability within the pH range from 4 to 10.

AF488 carboxylic acid is a non-reactive AF488 form that can be used as a reference standard in experiments where AF488 conjugates are used. The carboxylic acid can be also used for the synthesis of activated esters [such as sulfo-NHS, TFP (2,3,5,6-tetrafluorophenol) and STP (4-sulfo-2,3,5,6-tetrafluorophenol)] or modified with hydrazines, hydroxylamines, or amines in aqueous solutions using water-soluble carbodiimides. Thus, this derivative can be conjugated to molecules that contain amino groups, such as proteins, antibodies, and peptides. Therefore, AF488 carboxylic acid is used during solid-phase peptide synthesis for peptide modification in situ in the presence of activating agents such as HATU.

AF488 是一种亮绿色荧光染料,因其光稳定性而常用于显微镜检查和细胞分析。 AF488 可与 DAPI 一起使用,非常适合多重检测。这种高量子产率染料在 4 到 10 的 pH 值范围内具有荧光稳定性。

AF488 羧酸是一种非反应性 AF488 形式,可在使用 AF488 偶联物的实验中用作参考标准。该羧酸还可用于合成活性酯 [例如磺基-NHS、TFP (2,3,5,6-四氟苯酚) 和 STP (4-磺基-2,3,5,6-四氟苯酚)]或使用水溶性碳二亚胺在水溶液中用肼、羟胺或胺改性。因此,这种衍生物可以与含有氨基的分子结合,例如蛋白质、抗体和肽。因此,AF488 羧酸用于固相肽合成过程中,在 HATU 等活化剂存在的情况下进行原位肽修饰。

AF488 carboxylic acid相关产品

AF568 carboxylic acid

Non-reactive form of AF568 fluorescent dye

AF568 荧光染料的非反应性形式

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AF488 NHS ester

AF488 NHS ester, a bright, photostable and hydrophilic fluorescent dye for green channel.

AF488 NHS 酯,一种用于绿色通道的明亮、光稳定性和亲水性荧光染料。

General properties

Appearance: orange crystals  橙色晶体
Molecular weight: 648.74
Molecular formula: C21H11N2K3O11S2
IUPAC name: 4-(6-amino-3-iminio-4,5-disulfonato-3H-xanthen-9-yl)isophthalate
Solubility: good in DMSO, DMF
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.

储存:收到后 12 个月,在 -20°C 避光保存。 运输:在室温下长达 3 周。 避免长时间暴露在光线下。

MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 495
ε, L⋅mol−1⋅cm−1: 71800
Emission maximum, nm: 519
Fluorescence quantum yield: 0.91
CF260: 0.16
CF280: 0.10