Sulfo-Cyanine3 hydrazide

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Sulfo-Cyanine3 hydrazide

标签:Hydrazides, Microscopy, sulfo-Cyanine3,Cy3
货号 规格 价格 货期
11370 1 mg 110.00$ in stock
21370 5 mg 290.00$ in stock
41370 25 mg 690.00$ in stock
51370 50 mg 1270.00$ in stock
61370 100 mg 1990.00$ in stock

Sulfo-Cyanine3 is a sulfated derivative of Cyanine3 dye, well soluble in water because of two negatively charged sulfo groups in its structure. By its spectral characteristics, this yellow-orange fluorescent dye is an analog of Cy™3.

Hydrazides efficiently react with aldehydes and ketones resulting in hydrazones, so this compound can be used for conjugation with carbonyl derivatives of biomolecules.

The reaction runs in aqueous conditions, which is important when working with antibodies and proteins. Cys-diol groups in sugars in glycosylated proteins and antibodies can be oxidized into dialdehydes, and cysteine in proteins can be converted with enzymes to formyl glycerol (i. e. reactive groups for conjugation with Sulfo-Cyanine3 hydrazide). Carboxyl groups of aspartic and glutamic acids in proteins and peptides can be also conjugated with Sulfo-Cyanine3 hydrazide in the presence of activating agents: carbodiimide (EDAC) or methyl morpholine (DMTMM) derivatives.

Sulfo-Cy3 是 Cy3 染料的硫酸化衍生物,由于其结构中有两个带负电荷的磺基,因此可很好地溶于水。根据其光谱特性,这种橙黄色荧光染料是 Cy™3 的类似物。


反应在水性条件下进行,这在处理抗体和蛋白质时很重要。糖基化蛋白质和抗体中的糖中的 Cys-二醇基团可被氧化成二醛,蛋白质中的半胱氨酸可被酶转化为甲酰基甘油(即与磺基花青 3 酰肼结合的反应基团)。蛋白质和肽中的天冬氨酸和谷氨酸的羧基也可以在活化剂存在的情况下与磺基花青 3 酰肼结合:碳二亚胺 (EDAC) 或甲基吗啉 (DMTMM) 衍生物。

Absorption and emission spectra of sulfo-Cyanine3

Sulfo-Cyanine3 hydrazide

Sulfo-Cyanine3 hydrazide相关产品

Sulfo-Cyanine3 NHS ester

Water soluble sulfo-Cyanine3 SE activated ester for amino-biomolecule labeling.

用于氨基生物分子标记的水溶性磺基-Cy3 SE 活化酯。

Cyanine3 hydrazide

Cyanine3 hydrazide is a carbonyl-reactive dye for Cy3 channel.

Cy3 hydrazide 是 Cy3 通道的羰基反应性染料。

General properties

Appearance: dark red solid 暗红色固体
Molecular weight: 630.78
CAS number: 2144762-62-7
Molecular formula: C30H38N4O7S2
IUPAC name: 3H-​Indolium, 2-​[3-​[1-​(6-​hydrazinyl-​6-​oxohexyl)​-​1,​3-​dihydro-​3,​3-​dimethyl-​5-​sulfo-​2H-​indol-​2-​ylidene]​-​1-​propen-​1-​yl]​-​1,​3,​3-​trimethyl-​5-​sulfo-​, inner salt
Solubility: soluble in water, DMF, DMSO
Quality control: NMR 1H, HPLC-MS (90%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.

储存:收到后 24 个月,在 -20°C 避光保存。 运输:在室温下长达 3 周。 避免长时间暴露在光线下。

MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 548
ε, L⋅mol−1⋅cm−1: 162000
Emission maximum, nm: 563
Fluorescence quantum yield: 0.1
CF260: 0.03
CF280: 0.06
Cy™ is a registered trademark of Cytiva in some countries.