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- Sulfo-Cyanine5 maleimide
Sulfo-Cyanine5 maleimide
| 货号 | 规格 | 价格 | 货期 |
|---|---|---|---|
| 13380 | 1 mg | 110.00$ | in stock |
| 23380 | 5 mg | 290.00$ | in stock |
| 43380 | 25 mg | 690.00$ | in stock |
| 53380 | 50 mg | 1270.00$ | in stock |
| 63380 | 100 mg | 1990.00$ | in stock |
A water soluble, hydrophilic sulfo-Cyanine5 maleimide (Cy5® maleimide analog). We recommend this product for protein labeling, including labeling of antibodies as a perfect replacement for Cy5® maleimide. Labeled proteins can be easily separated from unreacted dye by gel filtration, spin column purification, dialysis, electrophoresis or chromatography.
An analog without sulfo groups, Cyanine5 maleimide, is also available.
水溶性、亲水性磺基-Cyanine5 马来酰亚胺（Cy5® 马来酰亚胺类似物）。 我们推荐该产品用于蛋白质标记，包括标记抗体作为 Cy5® 马来酰亚胺的完美替代品。 通过凝胶过滤、旋转柱纯化、透析、电泳或色谱法，可以很容易地将标记的蛋白质与未反应的染料分离。
也可使用不含磺基的类似物 Cyanine5 马来酰亚胺。
Sulfo-Cyanine5 absorbance and emission spectra
Sulfo-Cyanine5 maleimide相关产品
TAMRA azide, 6-isomer
Pure 6-isomer of tetramethylrhodamine (TAMRA) dye, azide derivative for copper-catalyzed and copper-free Click chemistry reactions with alkynes.
纯 6 异构体的四甲基罗丹明 (TAMRA) 染料，叠氮化物衍生物，用于与炔烃的铜催化和无铜点击化学反应。
ROX NHS ester, 6- isomer
Pure isomer 6-ROX NHS ester for amine labeling.
用于胺标记的纯异构体 6-ROX NHS 酯。
Sulfo-Cyanine5 amine
Sulfonated Cyanine5 amine derivative, for the conjugation with electrophiles, and enzymatic transamination labeling.
磺化的 Cy5 胺衍生物，用于与亲电试剂偶联和酶促转氨标记。
General properties
| Appearance: | dark blue powder 深蓝色粉末 |
| Molecular weight: | 803.00 |
| CAS number: | 2242791-82-6 |
| Molecular formula: | C38H43KN4O9S2 |
| Solubility: | soluble in water, DMSO, DMF |
| Quality control: | NMR 1H, HPLC-MS (95+%) |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
储存：收到后 24 个月，在 -20°C 避光保存。 运输：在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。 |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 646 |
| ε, L⋅mol−1⋅cm−1: | 271000 |
| Emission maximum, nm: | 662 |
| Fluorescence quantum yield: | 0.28 |
| CF260: | 0.04 |
| CF280: | 0.04 |
相关文献：
- Ast, J.; Novak, A.N.; Podewin, T.; Fine, N.H.F.; Jones, B.; Tomas, A.; Birke, R.; Roßmann, K.; Mathes, B:; Eichhorst, J.; Lehmann, M.; Linnemann, A.K.; Hodson, D.J.; Broichhagen, J. An expanded LUXendin color palette for GLP1R detection and visualization in vitro and in vivo. ChemRxiv, preprint. doi: 10.33774/chemrxiv-2021-7rngq
- Delvaux, N.A.; Mathew, B.; Rice, K.G. Fluorescent Labeling of Plasmid DNA for Gene Delivery: Implications of Dye Hydrophobicity on Labeling Efficiencies and Nanoparticle Size. Analytical Biochemistry, in press. doi: 10.1016/j.ab.2020.113895
- Bauer, B.W.; Davidson, I.F.; Canena, D.; Wutz, G.; Tang, W.; Litos, G.; Horn, S.; Hinterdorfer, P.; Peters, J.-M. Cohesin mediates DNA loop extrusion by a “swing and clamp” mechanism. Cell, 2021, 184(21), 5448–5464.e22. doi: 10.1016/j.cell.2021.09.016
- Hurley, C.M.; Kraut, D.A. Determination of Proteasomal Unfolding Ability. Methods in Molecular Biology, 2021, 2365, 217–244. doi: 10.1007/978-1-0716-1665-9_12