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- Reactive dyes
- Dye maleimides
- Cyanine3 maleimide
Cyanine3 maleimide
| 货号 | 规格 | 价格 | 货期 |
|---|---|---|---|
| 11080 | 1 mg | 110.00$ | in stock |
| 21080 | 5 mg | 210.00$ | in stock |
| 41080 | 25 mg | 410.00$ | in stock |
| 51080 | 50 mg | 695.00$ | in stock |
| 61080 | 100 mg | 1190.00$ | in stock |
Thiol mono-reactive Cyanine3 dye. This reagent can be used to attach Cyanine3 fluorophore (an analog of Cy3®) to proteins and peptides containing cysteine residues, as well as to other thiolated molecules (such as thiol-containing oligonucleotides).
Cystines should be reduced with TCEP (tris-carboxyethylphosphine) or other appropriate reductant prior to the labeling.
Labeling with Cyanine3 maleimide is selective, and efficient.
We recommend using water-soluble Sulfo-Cyanine3 maleimide for the labeling of antibodies and other sensitive proteins.
硫醇单反应性 Cyanine3 染料。 该试剂可用于将 Cyanine3 荧光团（Cy3® 的类似物）连接到含有半胱氨酸残基的蛋白质和肽以及其他硫醇化分子（例如含硫醇的寡核苷酸）上。
标记前应使用 TCEP（三羧乙基膦）或其他合适的还原剂还原胱氨酸。
用 Cyanine3 马来酰亚胺标记具有选择性且有效。
我们建议使用水溶性 Sulfo-Cyanine3 马来酰亚胺标记抗体和其他敏感蛋白。
Cy3 absorbance and emission spectra
Cyanine3 maleimide相关产品
Cyanine3 NHS ester
Amine-reactive Cyanine3 dye NHS ester.
胺反应性 Cy3 染料 NHS 酯。
Sulfo-Cyanine3 carboxylic acid
Sulfo-Cyanine3 free unactivated monofunctional carboxylic acid, water-soluble dye.
Sulfo-Cyanine3 不含未活化的单官能羧酸，水溶性染料。
Cyanine3 carboxylic acid
Free Cyanine3 carboxylic acid.
游离Cy3羧酸。
General properties
| Appearance: | red powder 红色粉末 |
| Molecular weight: | 666.56 |
| Molecular formula: | C36H43N4O3BF4 |
| Solubility: | well soluble in DMSO (0.50 M = 330 g/L), in DMF, dichloromethane, very poorly soluble in water (0.57 mM = 420 mg/L) |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
储存：收到后 24 个月，在 -20°C 避光保存。 运输：在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。 |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 555 |
| ε, L⋅mol−1⋅cm−1: | 150000 |
| Emission maximum, nm: | 570 |
| Fluorescence quantum yield: | 0.31 |
| CF260: | 0.04 |
| CF280: | 0.09 |
相关文献：
- Yang, M.; Zhang, L.; Sun, R.; Zhong, W.; Yang, Y.; Chen, C. Streptococcus pyogenes Cas9 displays biased one-dimensional diffusion on dsDNA to search for a target. bioRxiv, preprint. doi: 10.1101/754564
- Budiardjo, S.J.; Deay, J.J.; Calkins, A.L.; Wimalasena, V.K.; Montezano, D.; Biteen, J.S.; Slusky, J.S.G. Colicin E1 Fragments Potentiate Antibiotics by Plugging TolC. bioRxiv, preprint. doi: 10.1101/692251
- Mohale, M.; Gundampati, R.K.; Thallapuranam, S.; Heyes, C.D. Site-specific labeling and functional efficiencies of human fibroblast growth Factor-1 with a range of fluorescent dyes in the flexible N-Terminal region and a rigid β-turn region. Analytical Biochemistry, 2022, 640, 114524. doi: 10.1016/j.ab.2021.114524
- Bagert, J.D.; Mitchener, M.M.; Patriotis, A.L.; Dul, B.E.; Wojcik, F.; Nacev, B.A.; Feng, L.; Allis, C.D.; Muir, T.W. Oncohistone mutations enhance chromatin remodeling and alter cell fates. Nature Chemical Biology, 2021, 17(4), 403–411. doi: 10.1038/s41589-021-00738-1