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• Sulfo-Cyanine3.5 alkyne

Sulfo-Cyanine3.5 alkyne

Sulfo-Cyanine3.5 is a dye with fluorescence in the orange spectrum range (absorption maximum at 576 nm; emission maximum at 603 nm). Its absorption and emission spectra are between those of Cyanine3 and Cyanine5. Therefore, Sulfo-Cyanine3.5 is commonly used as a FRET-donor in a pair with Cyanine5, as a FRET-acceptor in a pair with Cyanine3, and in BRET experiments.

Sulfo-Cyanine3.5 is a sulfonated derivative with four sulfo groups, which allows its high hydrophilicity and performing all reactions with this reagent in aqueous solutions without adding organic solvents.

The reagent is an alkyne derivative of sulfo-Cyanine3.5 dye for copper-catalyzed Click chemistry. The terminal alkynyl (acetylene) group allows conjugating the fluorophore with the molecules containing an azide group through 1,3-dipolar cycloaddition in the presence of a copper(I) catalyst.

Sulfo-Cyanine3.5 是一种在橙色光谱范围内具有荧光的染料（最大吸收波长为 576 nm；最大发射波长为 603 nm）。 其吸收和发射光谱介于花青 3 和花青 5 之间。 因此，Sulfo-Cyanine3.5 通常与 Cyanine5 成对用作 FRET 供体，与 Cyanine3 成对用作 FRET 受体，并用于 BRET 实验。

Sulfo-Cyanine3.5 是一种具有四个磺基的磺化衍生物，具有高亲水性，可在不添加有机溶剂的情况下在水溶液中与该试剂进行所有反应。

该试剂是用于铜催化点击化学的磺基-Cyanine3.5 染料的炔烃衍生物。 末端炔基（乙炔）基团允许在铜 (I) 催化剂存在下通过 1,3-偶极环加成将荧光团与含有叠氮基的分子缀合。