AF488 azide荧光染料

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AF488 azide

标签:Azides AF488
货号 规格 价格 货期
11830 1 mg 110.00$ in stock
21830 5 mg 290.00$ in stock
41830 25 mg 690.00$ in stock
51830 50 mg 1270.00$ in stock
61830 100 mg 1990.00$ in stock

AF488 is a fluorescent dye. AF488 is a fluorophore pH-insensitive over a broad pH range (from 4 to 10). It has an absorption maximum at 495 nm and an emission maximum at 519 nm in the green spectrum region. The dye is hydrophilic and can be used to introduce the fluorescent label into various molecules, including proteins and antibodies. Conjugates of molecules with AF488 have high brightness and photostability and are commonly used in flow cytometry and microscopy. This allows the detection of biological objects with high sensitivity at a longer imaging time.

AF488 azide interacts with alkynyl derivatives of biomolecules in Click Chemistry reactions either in the presence of copper (I) catalyst (with terminal alkynes) or without catalyst (with cyclooctynes), leading to the formation of stable adducts.

AF488 是一种荧光染料。 AF488 是一种在宽 pH 范围(从 4 到 10)上对 pH 不敏感的荧光团。 它在绿色光谱区域具有 495 nm 的最大吸收和 519 nm 的最大发射。 该染料是亲水的,可用于将荧光标记引入各种分子,包括蛋白质和抗体。 分子与 AF488 的结合物具有高亮度和光稳定性,通常用于流式细胞术和显微镜检查。 这允许在更长的成像时间以高灵敏度检测生物对象。

AF488 叠氮化物在点击化学反应中与生物分子的炔基衍生物相互作用,无论是在铜 (I) 催化剂(带有末端炔烃)或没有催化剂(带有环辛炔)的情况下,都会导致形成稳定的加合物。

AF488 azide AF488 azide AF488 azide AF488 azide

Absorption and emission spectra of AF488

AF488 azide

AF488 azide相关产品

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用氨基标记的三磷酸尿苷,用于间接非放射性酶促 RNA 标记。

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Water-soluble Cy 5 succinimide ester.
水溶性 Cy 5 琥珀酰亚胺酯。

General properties

Appearance: orange solid
Mass spec M+ increment: 616.1
Molecular weight: 825.01
CAS number: 1679326-36-3 (with azidohexyl group)
Molecular formula: C36H56N8O10S2
Solubility: good in water, DMF, DMSO
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.储存:收到后 24 个月,在 -20°C 避光保存。 运输:在室温下长达 3 周。 避免长时间暴露在光线下。
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 495
ε, L⋅mol−1⋅cm−1: 71800
Emission maximum, nm: 519
Fluorescence quantum yield: 0.91
CF260: 0.16
CF280: 0.10