lumiprobe – Pyrenebutyric acid NHS ester

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Pyrenebutyric acid NHS ester

标签:NHS esters Protein labeling Pyrene
Cat. # Quantity Price Lead time
63520 100 mg 110.00$ in stock
 

Pyrene is a polyaromatic hydrocarbon which exhibits fluorescence in blue range of the spectrum. When two pyrene residues are closely spaced, excimer fluorescence is observed at longer wavelength. Therefore, pyrene is useful as a proximity label.

Pyrene exhibits affinity to graphite, and can be used as an anchor group for graphite. This reagent can be conjugated with biomolecules for their subsequent immobilization on graphite surfaces.

This NHS ester allows to label biomolecules with amino groups, such as proteins, peptides, amino-DNA and other molecules.

Pyrene is also a FRET donor for perylene.

芘是一种多环芳烃,在光谱的蓝色范围内显示荧光。 当两个芘残基间隔很近时,在较长波长下观察到准分子荧光。 因此,芘可用作邻近标签。

芘对石墨具有亲和力,可用作石墨的锚定基团。 该试剂可以与生物分子结合,以便随后将其固定在石墨表面上。

这种 NHS 酯允许用氨基标记生物分子,例如蛋白质、肽、氨基 DNA 和其他分子。

芘也是苝的 FRET 供体。

Absorption and emission spectra of pyrene fluorophore

Pyrenebutyric acid NHS ester

Pyrenebutyric acid NHS ester相关产品

ROX NHS ester, 5-isomer

Activated NHS ester of ROX (rhodamine X or rhodamine 101), pure 5-isomer, for the labeling of amine groups of biomolecules.

ROX 的活化 NHS 酯(罗丹明 X 或罗丹明 101),纯 5-异构体,用于标记生物分子的胺基。

Azidobutyric acid NHS ester

Activated ester for azido labeling of peptides and proteins.

用于肽和蛋白质的叠氮基标记的活化酯。

ROX azide, 5- isomer

ROX (aka rhodamine X or rhodamine 101) azide for biomolecule labeling through Click Chemistry.

ROX(又名罗丹明 X 或罗丹明 101)叠氮化物,用于通过 Click Chemistry 进行生物分子标记。

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General properties

Appearance: off-white solid
Mass spec M+ increment: 270.1
Molecular weight: 385.41
CAS number: 114932-60-4
Molecular formula: C24H19NO4
IUPAC name: 1-​Pyrenebutanoic acid, 2,​5-​dioxo-​1-​pyrrolidinyl ester
Solubility: good in DCM, chloroform, moderate in DMF, DMSO, low in water
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 343; 326; 313; 276; 265; 242; 234
Emission maximum, nm: 377; 397

参考文献:

  1. Nekrasov, N.; Jaric, S.; Kireev, D.; Emelianov, A.V.; Orlov, A.V.; Gadjanski, I.; Nikitin, P.I.; Akinwande, D.; Bobrinetskiy, I. Real-time detection of ochratoxin A in wine through insight of aptamer conformation in conjunction with graphene field-effect transistor. Biosensors and Bioelectronics, 2022, 200, 113890. doi: 10.1016/j.bios.2021.113890

Pyrenebutyric acid NHS ester