- Products
- Reactive dyes
- Dye NHS esters
- BDP FL NHS ester
BDP FL NHS ester
| 货号 | 规格 | 价格 | 货期 |
|---|---|---|---|
| 11420 | 1 mg | 110.00$ | in stock |
| 21420 | 5 mg | 210.00$ | in stock |
| 41420 | 25 mg | 410.00$ | in stock |
| 51420 | 50 mg | 695.00$ | in stock |
| 61420 | 100 mg | 1190.00$ | in stock |
BDP FL NHS ester is an advanced dye for 488 nm channel, a replacement for fluorescein, a molecule identical to BODIPY FL® NHS ester. An amino-reactive dye for the labeling of proteins and peptides.
While the absorbance and emission spectra of this molecule stay within FAM excitation and emission channels, this dye provides much better photostability, and outstanding brightness. The fluorescence spectrum of BODIPY-FL is narrower than that of FAM. This provides a better brightness for monochromator based instruments, when emission wavelength can be tuned to dye maximum.
The dye is neutral, possesses low molecular weight, and retains high quantum yield in conjugates.
The dye is a good replacement for fluorescein (FAM), BODIPY-FL, DyLight 488, Cy2, and other 488 nm dyes.
BDP FL NHS 酯是一种用于 488 nm 通道的高级染料，是荧光素的替代品，荧光素是一种与 BODIPY FL® NHS 酯相同的分子。 一种用于标记蛋白质和肽的氨基活性染料。
虽然这种分子的吸收和发射光谱停留在 FAM 激发和发射通道内，但这种染料提供了更好的光稳定性和出色的亮度。 BODIPY-FL 的荧光光谱比 FAM 窄。 这为基于单色器的仪器提供了更好的亮度，当发射波长可以调整到染料最大值时。
该染料是中性的，具有低分子量，并在偶联物中保持高量子产率。
该染料是荧光素 (FAM)、BODIPY-FL、DyLight 488、Cy2 和其他 488 nm 染料的良好替代品。
Absorption and emission spectra of BDP FL
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Cyanine5 azide 是一种用 Cyanine5（一种流行的荧光染料）进行 Click Chemistry 标记的试剂。
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General properties
| Appearance: | orange solid |
| Mass spec M+ increment: | 274.1 |
| Molecular weight: | 389.16 |
| CAS number: | 146616-66-2 |
| Molecular formula: | C18H18BF2N3O4 |
| Solubility: | Good in organic solvents (DMF, DMSO, dichlromethane), limited in water |
| Quality control: | NMR 1H, HPLC-MS (95+%) |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 503 |
| ε, L⋅mol−1⋅cm−1: | 92000 |
| Emission maximum, nm: | 509 |
| Fluorescence quantum yield: | 0.97 |
| CF260: | 0.015 |
| CF280: | 0.027 |
相关文献：
- Nascimento, A.; Pedro, M.N.S.; Pinto, I.F.; Aires-Barros, M.R.; Azevedo, A.M. Microfluidics as a high-throughput solution for chromatographic process development – The complexity of multimodal chromatography used as a proof of concept. Journal of Chromatography A, 2021, 1658, 462618. doi: 10.1016/j.chroma.2021.462618
- Best, Q.A.; Haack, R.A.; Swift, K.M.; Bax, B.M.; Tetin, S.Y.; Hershberger, S.J. A Rainbow of Acridinium Chemiluminescence. Luminescence, 2021, 36(4), 1097–1106. doi: 10.1002/bio.4038
- González, M.I.; González-Arjona, M.; Santos-Coquillat, A.; Vaquero, J.; Vázquez-Ogando, E.; de Molina, A.; Peinado, H.; Desco, M.; Salinas, B. Covalently Labeled Fluorescent Exosomes for In Vitro and In Vivo Applications. Biomedicines, 2021, 9, 81. doi: 10.3390/biomedicines9010081
- Volz, J.; Kusch, C.; Beck, S.; Popp, M.; Vögtle, T.; Meub, M.; Scheller, I.; Heil, H.S.; Preu, J.; Schuhmann, M.K.; Hemmen, K.; Premsler, T.; Sickmann, A.; Heinze, K.G.; Stegner, D.; Stoll, G.; Braun, A.; Sauer, M.; Nieswandt, B. BIN2 orchestrates platelet calcium signaling in thrombosis and thrombo-inflammation. Journal of Clinical Investigation, 2020, 130(11), 6064–6079. doi: 10.1172/JCI136457