半糖粒是细胞与细胞外基质之间的黏附结构。它们在将中间纤维锚定在细胞外基膜上发挥作用。在结构上，半脂质体有两种形式:I型和II型。I型半脂质体发育于分层上皮，如表皮。其主要成分包括细胞内连接蛋白果胶和BPAG1，粘附受体整合素α6β4和胶原型BP180/XVII。II型半脂质体作为I型半脂质体的简化形式出现在血管、雪旺细胞和消化道上皮中，仅由果胶和整合素α6β4组成。半糖体粘附受体通常与基底膜上的层粘连蛋白5相关。此外，层粘连蛋白5(其中层粘连蛋白2是一个亚基)通过VII型胶原蛋白与真皮层中的胶原纤维相连。半脂糖体相关蛋白的遗传缺失导致各种形式的大疱性表皮松解症，突出了它们在促进表皮和基底膜之间粘附的重要性。
果胶将中间丝与微管和微丝相连，并将中间丝固定在桥粒或半桥粒上。也可以将肌动蛋白等肌肉蛋白结合到肌肉的膜复合物上。可能不仅与丝状网络有关，而且与丝状网络的动态调节有关。肌肉的结构组成部分。异构体9在维持肌纤维完整性方面起着重要作用。
Cosmo Bio抗体,Cosmo Bio,Anti Plectin (PCN/PLTN) mAb (Clone PN753),CAC-NU-01-PLN
Application: IP, IF, WB
Clonality: Monoclonal
Host: Mouse
Purification: Supernatant
Reactivity: Porcine, Rabbit, Rat, Human
Hemidesmosomes are adhesive structures between cells and the extracellular matrix. They play a role in anchoring intermediate fibers to the extracellular basement membrane. Structurally, hemidesmosomes occur in two forms: Type I and Type II. Type I hemidesmosomes develop in stratified epithelia such as the epidermis. Its main components include the intracellular linker proteins Plectin and BPAG1, the adhesion receptor integrin α6β4 and collagen type BP180/XVII. Type II hemidesmosomes occur in blood vessels, Schwann cells, and digestive tract epithelia as a simplified form of Type I hemidesmosomes, consisting only plectin and integrin α6β4. The hemidesmosomal adhesion receptor is normally associated with Laminin 5 in the basement membrane. Furthermore, Laminin 5 (of which Laminin gamma 2 is a subunit) is linked to collagen fibers in the dermis via type VII collagen. Genetic deletion of hemidesmosome-related proteins causes various forms of epidermolysis bullosa, highlighting their importance in promoting adhesion between the epidermis and the basement membrane.
Plectin interlinks intermediate filaments with microtubules and microfilaments and anchors intermediate filaments to desmosomes or hemidesmosomes. Could also bind muscle proteins such as actin to membrane complexes in muscle. May be involved not only in the filaments network, but also in the regulation of their dynamics. Structural component of muscle. Isoform 9 plays a major role in the maintenance of myofiber integrity.
References:
1) Yamauchi T., et al. J. Dermatol. Sci., 76:25-33 (2014).
2) Hirako Y., et al. Exp. Cell Res., 324:172-182 (2014).
3) Hirako Y., et al. J. Biol. Chem., 273:9711-9717 (1998).