Precast Gel,Mini Gel 8x9cm,U-PAGEL,atto代理,atto预制凝胶

目的和应用

最适合分离高分子量蛋白质

1500 kDa蛋白的分离和检测(UH-T/R310)

sds – page

Native-PAGE

筛选

蛋白质印迹电泳(WB)

DNA(如PCR产物)电泳

特性
适用于500 kDa以上聚合物蛋白的分离(UH-T/R5, UH-T/R310)

5~600kDa宽分数范围(UH-T/R420)

物理强度增加,即使是低浓度的凝胶也很难撕裂

长期保质期1年(自生产之日起)

可实现高速、短时间(30min~)迁移

聚合物印迹法也很好

广泛应用(SDS-PAGE, Native-PAGE, DNA)

tris -甘氨酸(-SDS)可作为迁移缓冲剂

Code No. Description unit
2331300 UH-T5 u-PAGEL H 5%14-well 10 gels 1 pk
2331310 UH-R5 u-PAGELH 5%18-well 10 gels 1 pk
2331302 uH-T310u-PAGELH 3~10%14-well 10 gels 1 pk
2331312 UH-R310 u-PAGEL H 3~10%18-well 10 gels 1 pk
2331304 UH-T420 u-PAGELH 4~20%14-well 10 gels 1 pk
2331314 UH-R420 u-PAGEL H4~20%/18-well 10 gels 1pk

Polyacrylamide gel is a polymer in which acrylamide and N, N’-Methylenebisacrylamide (Bis), a crosslinking agent, are bonded by radical polymerization. Acrylamide forms a linear structure and the crosslinking agent acts as a bridge to form a polymer (gel) with a net structure. The lower the gel concentration, the larger the pore size, so high molecular protein separation is possible. However, Bis has low solubility, so it becomes a non-uniform gel due to clumps. Non-uniform gels fall in strength and tear easily. Therefore, the problem was that it was difficult to handle.
This time, ATTO succeeded in developing a uniform gel at low concentrations using a new crosslinking agent with higher solubility and longer bonding arms than Bis. It has excellent strength with tensile fracture stress about 1.7 times higher than Bis. In addition, with large pore size, the sample smoothly enters the gel’s net structure without clogging, showing higher separability.

聚丙烯酰胺凝胶是由丙烯酰胺与交联剂N, N’-亚甲基双丙烯酰胺(Bis)通过自由基聚合结合而成的聚合物。丙烯酰胺形成线性结构,交联剂作为桥梁形成具有网状结构的聚合物(凝胶)。凝胶浓度越低,孔隙越大,因此可以分离高分子蛋白。Bis的溶解度较低,由于结块而成为不均匀的凝胶。不均匀的凝胶强度下降,容易撕裂。因此,问题是它很难处理。
这一次,ATTO成功地开发了一种低浓度的均匀凝胶,使用了一种新的交联剂,具有比Bis更高的溶解度和更长的键臂。它具有优异的强度,拉伸断裂应力约为Bis的1.7倍。此外,样品孔径大,能顺利进入凝胶的网状结构而不堵塞,具有较高的可分离性。