lumiprobe DiO, lipophilic tracer 亲脂性示踪剂(神经元示踪剂)

lumiprobe DiO, lipophilic tracer 亲脂性示踪剂(神经元示踪剂)

Cell biology Tracers Microscopy
Cat. # Quantity Price Lead time
2936-5mg 5 mg   21 days
2936-50mg 50 mg 159.00$ 21 days

DiO, also called DiOC18(3), is a green fluorescent, lipophilic carbocyanine dye. DiO’s excitation max is 484 nm, and emission max is 501 nm.

DiO is widely used as an anterograde and retrograde neuronal tracer in living and fixed tissues and cells. DiO uniformly labels neurons via diffusion in the plasma membrane. In intact tissue, the dye does not transfer from labeled to unlabeled cells but some transfer may occur when the membrane is disrupted, for example after sectioning.

DiO and DiI are often used together in dual-color studies. But DiO has a slower lateral diffusion rate on the membranes than that of DiI.

DiO,也称为 DiOC18(3),是一种绿色荧光、亲油性羰花青染料。DiO 的最大激发波长为 484 nm,最大发射波长为 501 nm。

DiO 被广泛用作活的和固定的组织和细胞中的顺行和逆行神经元示踪剂。DiO 通过质膜中的扩散均匀地标记神经元。在完整组织中,染料不会从标记的细胞转移到未标记的细胞,但当膜被破坏时,例如在切片后,可能会发生一些转移。

DiO 和 DiI 在双色研究中经常一起使用。但 DiO 在膜上的横向扩散速率比 DiI 慢。

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General properties

Molecular weight: 782.27
Molecular formula: C53H85N2O2+
Quality control: NMR 1H and HPLC-MS (95+%)
Storage conditions: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
MSDS: Download
Product specifications

DiO, lipophilic tracer

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