Grace: Vydac

Vydac TP

Vydac TP was the first range of wide pore media phases developed in the Vydac range and became the industry standard for peptide and protein separations. The large pores of the 300A TP silica give polypeptide molecules complete access to the interior of the silica pores.

  

The general features of Vydac TP include:

• Long column lifetime and negligible phase leaching
• Reliable protein purifications, scalable from analytical to preparative scale
• Referenced in a large number of patents and publications

 

 Vydac TP phase specifications

Vydac TP Phase	Functional group	Particle size (um)	Chromatographic Properties	Application/Benefit	USP Code
Vydac 218TP	C18	3, 5, 10	First generation polymeric C18 phase with unique selectivity	Small polypeptides 4-5K MW, enzymatic digest fragments, natural and synthetic peptides, multi-ring compounds	L1
Vydac 238TP	C18	5	First generation monomeric C18 phase	Use for same applications as 218TP, but offers different C18 selectivity	L1
Vydac 208TP	C8	5	Less hydrophobic than C18TP phase	Polypeptides 10-20K MW	L7
Vydac 214TP	C4	5,10	First generation C4 phase	Glycoproteins, haemoglobin variants, histones, insulin variants, membrane proteins	L26
Vydac 214ATP	C4	5	C4 phase with lower level of endcapping	Optimised for analysis of human growth hormone	L26
Vydac 219TP	Diphenyl	5,10	Lowest capacity first generation diphenyl phase	Polypeptides with aromatic side chains, large hydrophobic proteins, membrane-spanning peptides, lipid peptides, fusion proteins from inclusion bodies	L11
Vydac 201TP	C18	5, 10	Non-endcapped C18 phase	Developed for separation of PAHs	L1
Vydac 202TP	C18	5	Higher carbon load than 201TP	Developed for separation of PAHs	L1

 

Vydac 218TP and 238TP are used for small polypeptides of 4-5kDa, enzymatic digest fragments, natural and synthetic peptides and multi-ring compounds.

Vydac 208TP is recommended for polypeptides of molecular weight 10-20kDa.

Vydac 214TP is recommended for the analysis of glycoproteins, haemoglobin variants, histones, insulin variants and membrane proteins.

Vydac 219TP is suitable for polypeptides with aromatic side chains, large hydrophobic proteins, membrane-spanning peptides, lipid peptides and fusion proteins from inclusion bodies.

Vydac MS

Vydac MS columns are a later development from Grace for the reversed-phase HPLC separation of biomolecules. A proprietary surface treatment and bonding process give Vydac MS columns unique selectivity. A variety of reversed-phases makes this product line suitable for the analysis of small peptides to large intact, undenatured proteins.

The general features of Vydac MS include:

•  300A pore size spherical silica

•  Five reversed-phase chemistries

•  Excellent peak shape with little or no TFA

•  High protein recoveries make scale-up easy

Vydac MS Phase Specifications

Vydac MS Phase	Functional Group	Particle Size (um)	Chromatographic Properties	Application/Benefit	USP Code
Vydac 218MS	C18	5, 10	Polymeric bonding, highest hydrophobic interaction and unique geometric selectivity	Use for simple enzymatic digests (<12 proteins) or biomolecules 0 – 5k MW	L1
Vydac 238MS	C18	5	Monomeric bonding offers increased peptide interaction and generally yields higher peak counts	Use for same applications as 218MS, but offers different C18 selectivity	L1
Vydac 208MS	C8	5	Lower hydrophobicity is better for larger biomolecules	Ideal for biomolecules 5-10K MW	L7
Vydac 214MS	C4	5, 10	Lower capacity than C18 or C8, suitable for hydrophobic proteins or when minimal organic solvent is desired	Ideal for biomolecules >10K MW, undenatured intact proteins, antibodies, oligonucleotides, human growth hormone	L26
Vydac 219MS	Diphenyl	5	Lowest capacity, aromatic functional groups	Highly selective for proteins with aromatic side chains	L11

The improved selectivity for peptides on the Vydac MS columns results in better primary structure definition and easier identification of degradation products and other protein characteristics.
Vydac 218MS and 238MS are used for simple enzymatic digests (< 12 proteins) or biomolecules (0-5kDa).

Vydac 208MS is ideal for biomolecules of molecular weight 5-10kDa.

Vydac 214MS has lower capacity than C18 or C8 and is suitable for hydrophobic proteins or when minimal organic solvent is required. It is recommended for biomolecules of > 10kDa molecular weight, undenatured intact proteins, antibodies, oligonucleotides, human growth hormone.

Vydac 219MS has the lowest capacity and is highly selective for proteins with aromatic side chains.

Vydac Everest

Everest columns have unique selectivity and sensitivity, which are the result of bonding technology that improves C18 surface coverage and deactivates residual silanols. Previously, the best 300A C18 chemistries have had carbon coverage in the 2.8 to 3.6umol/m2 range. Everest C18 coverage is in excess of 4umol/m2 and approximates the theoretical limit based on surface area. The increased shielding of the base silica increases column lifetime and reduces the amount of TFA required to shield the silica.

The general features of Vydac Everest  include:

•  Unique selectivity for hydrophilic and hydrophobic peptides

•  High resolution for complex peptide digests

•  Excellent sensitivity with little or no TFA in mobile phase

Vydac Everest Phase Specifications

Vydac Everest Phase	Particle Size(um)	Chromatographic Properties	Application//Benefit	USP Code
Vydac 238EV (C18)	5, 10	Maximum surface coverage for highest resolution of complex samples	Complex enzymatic digests (>12 proteins)	L1

Everest columns are available in nano/capillary to preparative scale dimensions.

Vydac Denali

Vydac Denali (238DE) is a 120A C18 bonded phase with high carbon coverage, suitable for the analysis of both acidic and basic analytes. It has applications for small molecule analyses of interest to pharmaceutical and environmental laboratories.

The general features of Vydac Denali include:

•  High retentivity

•  LC-MS of small molecules

•  Fully scalable from capillary to process

Vydac 302IC Anion-Exchange

Vydac 302IC is a low capacity anion-exchange (quaternary amine) material based on a high purity 10um large pore size silica.

• Well referenced for  ‘non-suppressed’ ion chromatography applications
• Used with standard HPLC systems for analysis of common ions
• Widely used for environmental analysis

For further technical information:

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