lumiprobe -Universal CPG type II, 1000A

Universal CPG type II, 1000A

Cat. # Quantity Price Lead time
2923-1g 1 g 45.00$

2923-10g 10 g 410.00$

Universal CPG type II, 1000A

Universal CPG type II, 1000A

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Items in stock will be shipped on 08.06.2022

Universal CPG type II, 1000A

Free express shipping from 300$!
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Universal CPG type II, 1000A

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Items in stock will be shipped on 08.06.2022

Universal CPG type II, 1000A

Free express shipping from 300$!
Add products for to your cart and get free express shipping to , ZIP .

Universal CPG type II, 1000A

Free express shipping from 300$!
Your cart total is 0$, and your order qualifies for free express shipping to , ZIP .

The Universal CPG type II, 1000A is one of universal supports used to immobilize nucleosides for synthesizing oligonucleotides and to increase rate of dephosphorylation of the 3′ end oligonucleotide during deblocking.

For the cleavage from the support and oligonucleotide deprotection anhydrous ammonia gas-phase, ammonium hydroxide/methylamine mixture and other basic reagents can be used in a short time. The Universal CPG type II, 1000A is suitable for use in harsh conditions and makes cleavage and deprotection faster compared to universal supports. Pore size of 1000 Å is recommended for the synthesis of oligonucleotides up to 120 bases.

Usage

Coupling: Standard conditions for universal CPG.

Deprotection: 2 hours at 80 °C or 8 hours at 55 °C using concentrated ammonia; 15 minutes at 65 °C using AMA mixture, ammonium hydroxide – 40% methylamine (1:1).

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Controlled pore glass (CPG) 1000 solid support for synthesis of oligonucleotides with 3’-terminal DusQ1 quencher. Compatible with standard deblocking conditions.

Oligo synthesis details

Pore size, Å: 1000
Typical loading, umol/g: 50−95
Coupling conditions: standard coupling, identical to normal nucleobases
Cleavage conditions: ammonium hydroxide 2 hours at 80 °C or 8 hours at 55 °C; AMA mixture, ammonium hydroxide – 40% methylamine (1:1), 15 minutes at 65 °C
Deprotection conditions: identical to protected nucleobases

Universal CPG type II, 1000A

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