1· High performance multiplex PCR；KAPA2G Fast Multiplex PCR Kits are based on a second- generation (2G) polymerase capable of synthesizing DNA faster than wild-type Taq and other DNA polymerases. A total extension time of 15 sec/cycle is sufficient for many multiplex assays, compared to 60 – 90 sec/cycle for competitor kits containing wild-type Taq
2·60% reduction in total cycling time；Fast PCR protocols using KAPA2G Fast Multiplex PCR Kits are based on reduced extension times that allow for up to 60% reduction in PCR cycling time, without the risk of compromising reaction performance, or having to invest in specialized PCR consumables or instrumentation
3·Uniform representation of all amplicons in 12-plex PCR；12-plex Multiplex PCR performed with the KAPA2G Fast Multiplex PCR Kit, Competitor Q and Competitor I. Achieving uniform representation of all amplicons in a complex multiplex assay is a challenge due to amplification bias, a result of differences in amplicon length, secondary structure and priming efficiency. Reactions (25 μl) contained 1X PCR Master Mix (KAPA and Competitor Q) or 1X PCR Buffer, 3 mM MgCl2, 0.2 mM of each dNTP and 1 U of hot start Taq DNA Polymerase (home brew multiplex reagents, with Competitor I). Human genomic DNA was used as template (250 ng – 2 ng per reaction), and primers were supplied at 0.2 μM each. Cycling was performed according to manufacturers’ recommendations (30 cycles).
4.Successful multiplex PCR with difficult, GC-rich targets6-plex GC-rich Multiplex PCR performed with the KAPA2G Fast Multiplex PCR Kit, Competitor Q and Competitor I. Successful Multiplex PCR with wild-type Taq is limited to easy, simple targets that can be amplified with equal efficiency. The improved processivity of the engineered KAPA2G Fast DNA Polymerase allows uniform multiplex PCR of a broad range of difficult targets. Reactions (25 μl) contained 1X PCR Master Mix (KAPA and Competitor Q) or 1X PCR Buffer, 3 mM MgCl2, 0.2 mM of each dNTP and 1 U of hot start Taq DNA Polymerase (home brew multiplex reagents, with Competitor I). Human genomic DNA was used as template (250 ng – 2 ng per reaction), and primers were supplied at 0.2 μM each. DMSO (5%) and KAPA Enhancer 1 (1X) was added to all reactions. Cycling was performed according to manufacturers’ recommendations (30 cycles). Amplicons range in size from 241 – 642 bp, and in GC content from 72.7 – 83.8%.
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DNA羟甲基化定量试剂盒(荧光法)48 次,A-P-1037-48DNA羟甲基化定量试剂盒(荧光法)96 次,A-P-1037-96 DNA羟甲基化定量试剂盒(比色法)48 次,A-P-1036-48DNA羟甲基化定量试剂盒(比色法)96 次,A-P-1036-96 DNA甲基化定量试剂盒(荧光法)48 次,A-P-1035-48DNA甲基化定量试剂盒(荧光法)96 次,A-P-1035-96 DNA甲基化定量试剂盒(比色法)48 次,A-P-1034-48DNA甲基化定量试剂盒(比色法)96 次,A-P-1034-96 通用DNA甲基化定量试剂盒48 次,A-P-1011-48通用DNA甲基化定量试剂盒96 次,A-P-1011-96 超敏DNA甲基化定量试剂盒48 次,A-P-1021-48超敏DNA甲基化定量试剂盒96 次,A-P-1021-96 总DNA甲基化定量试剂盒48 次,A-P-1014-48总DNA甲基化定量试剂盒96 次,A-P-1014-96