蛋白酶&蛋白质组分析,蛋白内切酶 LysC,NEB P8109S,NEB代理,New England Biolabs RNase

识别位点

特性
通过质谱进行蛋白质组学分析的理想用酶无蛋白酶污染
最适用于多肽的鉴定

概述
蛋白内切酶 LysC(flavastacin)是丝氨酸蛋白内切酶,克隆自产酶溶杆菌,能特异性切割赖氨酸羧基端的肽键。该酶是测序级酶适用于蛋白质组学和糖生物学应用。

注意:在 200 μl 双蒸水中重溶 LysC,制成 100 ng/μl 含10 mM Tris-HCl, pH 8.0 的溶液。溶液可以在 4℃ 储存几天,或者分装成一次性使用量于 -20℃ 储存几个月。
来源
提纯自产酶溶杆菌(Lysobacter enzymogenes)。

分子量
30,000 daltons。

重溶
蛋白内切酶 LysC 需溶解于 200 μl 双蒸水中制成 100 ng/μl 含10 mM Tris-HCl, pH 8.0 的溶液。快速自裂解效率随酶浓度不同而异。

M0280S Uracil-DNA Glycosylase (UDG) 1,000 units
M0280L Uracil-DNA Glycosylase (UDG) 5,000 units
M0281S Uracil Glycosylase Inhibitor (UGI) 200 units
M0281L 1,000 units
Uracil Glycosylase Inhibitor (UGI)
M0282S 1,000 units
APE 1
M0282L 5,000 units
APE 1
M0284S Multiplex PCR 5X Master Mix 100 rxns (50 µl vol)
M0285S Taq 5X Master Mix(50 µl vol) 100 rxns
M0285L Taq 5X Master Mix(50 µl vol) 500 rxns
M0287S LongAmp Taq 2X Master Mix(50 µl vol) 100 rxns
M0287L 500 rxns
LongAmp Taq 2X Master Mix(50 µl vol)
M0288S RNase HII 250 units
M0288L RNase HII 1,250 units
M0289V Antarctic Phosphatase 500 units
M0289S Antarctic Phosphatase 1,000 units
M0289L Antarctic Phosphatase 5,000 units
M0290V 500 units
Alkaline Phosphatase, Calf Intestinal (CIP)
M0290S Alkaline Phosphatase, Calf Intestinal (CIP) 1,000 units
M0290L 5,000 units
Alkaline Phosphatase, Calf Intestinal (CIP)
M0291S Tma Endonuclease III 500 units
M0291L Tma Endonuclease III 2,500 units
M0293V Exonuclease I (E. coli) 1,500 units
M0293S Exonuclease I (E. coli) 3,000 units
M0293L Exonuclease I (E. coli) 15,000 units
M0294S Tth Endonuclease IV 500 units
M0294L Tth Endonuclease IV 2,500 units
M0296S Thermostable Inorganic Pyrophosphatase 250 units
M0296L Thermostable Inorganic Pyrophosphatase 1,250 units
M0297S RNase H 250 units
M0297L RNase H 1,250 units
M0298S Cre Recombinase 50 units
M0298L Cre Recombinase 250 units
M0299S Endonuclease VIII 1,000 units
M0299L Endonuclease VIII 5,000 units
M0300S T4 Gene 32 Protein 100 µg
M0300L T4 Gene 32 Protein 500 µg
M0301S Topoisomerase I (E. coli) 100 units
M0301L Topoisomerase I (E. coli) 500 units
M0302S T7 Endonuclease I 250 units
M0302L T7 Endonuclease I 1,250 units
M0303S DNase I (RNase-free) 1,000 units
M0303L DNase I (RNase-free) 5,000 units
M0304S Endonuclease IV 1,000 units
M0304L Endonuclease IV 5,000 units
M0305S Endonuclease V 250 units
M0305L Endonuclease V 1,250 units
M0306S DNA Gyrase (E. coli) 100 units
M0306L 500 units
DNA Gyrase (E. coli)
M0307S RNase Inhibitor 2,000 units
M0307L RNase Inhibitor 10,000 units
M0308S T4 PDG (Endonuclease V) 2,000 units
M0308L T4 PDG (Endonuclease V) 10,000 units
M0309S PreCR Repair Mix 30 rxns
M0309L PreCR Repair Mix 150 rxns
M0310S p19 siRNA Binding Protein 1,000 units
M0310L p19 siRNA Binding Protein 5,000 units
M0313S Human Alkyladenine Glycosylase (hAAG) 500 units
M0313L Human Alkyladenine Glycosylase (hAAG) 2,500 units
M0314S Murine RNase Inhibitor 3,000 units
M0314L Murine RNase Inhibitor 15,000 units
M0315S Terminal Transferase 500 units
M0315L Terminal Transferase 2,500 units
M0320V Taq DNA Polymerase with Standard Taq (Mg-free) Buffer 200 units
M0320S Taq DNA Polymerase with Standard Taq (Mg-free) Buffer 400 units
M0320L Taq DNA Polymerase with Standard Taq (Mg-free) Buffer 2,000 units
M0321V Taq DNA Polymerase with ThermoPol II (Mg-free) Buffer 200 units
M0321S Taq DNA Polymerase with ThermoPol II (Mg-free) Buffer 400 units
M0321L Taq DNA Polymerase with ThermoPol II (Mg-free) Buffer 2,000 units
M0323V LongAmp Taq DNA Polymerase 250 units
M0323S LongAmp Taq DNA Polymerase 500 units
M0323L LongAmp Taq DNA Polymerase 2,500 units
M0324V Crimson Taq DNA Polymerase 100 units
M0324S Crimson Taq DNA Polymerase 200 units
M0324L Crimson Taq DNA Polymerase 1,000 units
M0325V Crimson Taq DNA Polymerase with (Mg-free) Buffer 100 units
M0325S Crimson Taq DNA Polymerase with (Mg-free) Buffer 200 units
M0325L Crimson Taq DNA Polymerase with (Mg-free) Buffer 1,000 units
M0326V Crimson LongAmp Taq DNA Polymerase 125 units
M0326S Crimson LongAmp Taq DNA Polymerase 250 units
M0326L Crimson LongAmp Taq DNA Polymerase 1,250 units
M0327V Sulfolobus DNA Polymerase IV 50 units
M0327S Sulfolobus DNA Polymerase IV 100 units
M0327L Sulfolobus DNA Polymerase IV 500 units
M0328V Bst DNA Polymerase, Full Length 250 units
M0328S Bst DNA Polymerase, Full Length 500 units
M0328L Bst DNA Polymerase, Full Length 2,500 units
M0330V Bsu DNA Polymerase, Large Fragment 100 units
M0330S Bsu DNA Polymerase, Large Fragment 200 units
M0330L Bsu DNA Polymerase, Large Fragment 1,000 units
M0331S 5´ Deadenylase 1,000 units
M0332V Hemo KlenTaq 100 rxns (25 µl vol)
M0332S Hemo KlenTaq 200 rxns (25 µl vol)
M0332L Hemo KlenTaq 1,000 rxns (25 µl vol)
M0333V Therminator III DNA Polymerase 100 units
M0333S Therminator III DNA Polymerase 200 units
M0333L Therminator III DNA Polymerase 1,000 units
M0334S Therminator γ DNA Polymerase 200 units
M0334L Therminator γ DNA Polymerase 1,000 units
M0335S hNEIL1 500 units
M0335L hNEIL1 2,500 units
M0336S hSMUG1 500 units
M0336L hSMUG1 2,500 units
M0337S Poly(U) Polymerase 60 units
M0338S XRN-1 20 units
M0392S b-Agarase I 100 units
M0392L b-Agarase I 500 units
M0393S Apyrase 10,000 milliunits
M0393L Apyrase 50,000 milliunits
M0394S ATP Sulfurylase 10 units
M0394L 50 units
ATP Sulfurylase
M2200V Quick Ligation Kit 15  rxns
M2200S Quick Ligation Kit 30  rxns
M2200L Quick Ligation Kit 150  rxns
M2401S ET SSB (Extreme Thermostable ssBinding Protein) 50 µg
M2402S Tth RecA 100 µg
M2403S Pyrophosphatase, inorganic (yeast) 10 units
M2403L Pyrophosphatase, inorganic (yeast) 50 units
M2501S Histone H1 Human, Recombinant 100 µg
M2502S Histone H2A Human, Recombinant 100 µg
M2503S Histone H3.1 Human, Recombinant 100 µg
M2504S Histone H4 Human, Recombinant 100 µg
M2505S Histone H2B Human, Recombinant 100 µg
M2506S Histone H3.2 Human, Recombinant 100 µg
M2507S Histone H3.3 Human, Recombinant 100 µg
M2551S TransPass R1 Transfection Reagent 0.4 ml
M2552S TransPass R2 Transfection Reagent 1.0 ml
M2553S 0.6 ml
TransPass D1 Transfection Reagent
M2553L TransPass D1 Transfection Reagent 3.0 ml
M2554S TransPass D2 Transfection Reagent 0.6 ml
M2554L TransPass D2 Transfection Reagent 3.0 ml
M2556S TransPass HeLa Transfection Reagent 2 x 0.4 ml
M2557S 1.2 ml
TransPass COS/293 Transfection Reagent
M2558S TransPass HUVEC Transfection Reagent 1.8 ml
M2561S TransPass V 0.6 ml
M2563S TransPass P Protein Transfection Reagent 0.125 ml
M5505S USER Enzyme 50 units
M5505L USER Enzyme 250 units
N0315S M13K07 Helper Phage 1.8 ml
N0316S M13KE  Phage 0.04 ml
N0340S Lambda Ladder PFG Marker 50 gel lanes
N0345S Yeast Chromosome PFG Marker 50 gel lanes
N0350S Low Range PFG Marker 50 gel lanes
N0356S dm5CTP 1 µmol
N0362S ssRNA Ladder 25 gel lanes
N0363S dsRNA Ladder 25 gel lanes
N0364S Low Range ssRNA Ladder 25 gel lanes
N0417S pLox2+ (linearized) 10 µg
N0445S 7-deaza-dGTP 0.15 µmol
N0446S Deoxynucleotide Solution Set 25 µmol of each
N0447V Deoxynucleotide Solution Mix 4 µmol of each
N0447S Deoxynucleotide Solution Mix 8 µmol of each
N0447L Deoxynucleotide Solution Mix 40 µmol of each
N0460S Acyclonucleotide Set 0.5 µmol of each
N0466S Ribonucleotide Solution Mix 8 µmol of each
N0466L Ribonucleotide Solution Mix 40 µmol of each
N0467V Quick-Load 100 bp DNA Ladder 37.5 gel lanes
N0467S Quick-Load 100 bp DNA Ladder 125 gel lanes
N0467L Quick-Load 100 bp DNA Ladder 375 gel lanes
N0468V Quick-Load 1 kb DNA Ladder 37.5 gel lanes
N0468S Quick-Load 1 kb DNA Ladder 125 gel lanes
N0468L Quick-Load 1 kb DNA Ladder 375 gel lanes
N0469V Quick-Load 2-log DNA Ladder (0.1-10.0 kb) 37.5 gel lanes
N0469S Quick-Load 2-log DNA Ladder (0.1-10.0 kb) 125 gel lanes
N0469L Quick-Load 2-log DNA Ladder (0.1-10.0 kb) 375 gel lanes
N0471S DNA Gyrase (E. coli) Substrate 20 µg
N0472S Supercoiled DNA Ladder 100 gel lanes
N1500S NEBlot Kit 25 rxns
N1500L NEBlot Kit 50 rxns
N1501S Octadeoxyribonucleotides in 10X Labeling Buffer 25 rxns
N1501L Octadeoxyribonucleotides in 10X Labeling Buffer 50 rxns
N2100S Fluorescein-siRNA Transfection Control 1 nmol
N2101S siRNA Marker 100 gel lanes
N2102S microRNA Marker 100 gel lanes
N3011S Lambda DNA 250 µg
N3011L Lambda DNA 1,250 µg
N3012S Lambda DNA-HindIII Digest 150 gel lanes
N3012L Lambda DNA-HindIII Digest 750 gel lanes
N3013S Lambda DNA (N6-methyladenine-free) 250 µg
N3013L Lambda DNA (N6-methyladenine-free) 1,250 µg
N3014S Lambda DNA-BstEII Digest 150 gel lanes
N3014L Lambda DNA-BstEII Digest 750 gel lanes
N3019S Lambda DNA-Mono Cut Mix 100 gel lanes
N3019L Lambda DNA-Mono Cut Mix 500 gel lanes
N3021S PhiX174 RF I DNA 30 µg
N3021L PhiX174 RF I DNA 150 µg
N3022S PhiX174 RF II DNA 30 µg
N3022L PhiX174 RF II DNA 150 µg
N3023S PhiX174 Virion DNA 50 µg
N3023L PhiX174 Virion DNA 250 µg
N3026S PhiX174 DNA-HaeIII Digest 50 gel lanes
N3026L PhiX174 DNA-HaeIII Digest 250 gel lanes
N3031S pBR322 DNA-BstNI Digest 50 gel lanes
N3031L pBR322 DNA-BstNI Digest 250 gel lanes
N3032S pBR322 DNA-MspI Digest 50 gel lanes
N3032L pBR322 DNA-MspI Digest 250 gel lanes
N3033S pBR322 Vector 50 µg
N3033L pBR322 Vector 250 µg
N3034S pKLAC1-GLuc Control Plasmid 20 µg
N3041S pUC19 Vector 50 µg
N3041L pUC19 Vector 250 µg