GLUTATHIONE SEPH 4 FF
| 17-5132-02 | GLUTATHIONE SEPH 4 FF, 100 ML |
| 17-5132-03 | GLUTATHIONE SEPH 4 FF, 500 ML |
| 17-5138-01 | RMP PROT A SEPH FF 5 ML |
| 17-5138-02 | RMP PROT A SEPH FF 25 ML |
| 17-5138-03 | RMP PROT A SEP FF 200 ML |
| 17-5199-01 | MABSELECT 25 ML |
| 17-5199-02 | MABSELECT 200 ML |
| 17-5268-01 | NI SEPHAROSE HP 25 ML |
| 17-5268-02 | NI SEPHAROSE HP 100 ML |
| 17-5269-02 | MABSELECT XTRA 200 ML |
| 17-5269-07 | MABSELECT XTRA 25ML |
| 17-5279-01 | GLUTATHIONE SEPHAROSE HP 25 ML |
| 17-5279-02 | GLUTATHIONE SEPH HP 100 ML |
| 17-5280-01 | NPROTEIN A SEPHAROSE 4 FF 5ML |
| 17-5280-02 | NPROTEIN A SEPH 4 FF 200 ML |
| 17-5280-04 | NPROTEIN A SEPHAROSE 4FF 25ML |
| 17-5318-01 | NI SEPHAROSE 6FF, 25 ML |
| 17-5318-02 | NI SEPHAROSE 6FF, 100 ML |
| 17-5318-03 | NI SEPHAROSE 6FF, 500 ML |
| 17-5318-06 | NI SEPHAROSE 6 FF 5 ML |
| 17-5438-01 | MABSELECT SURE 25 ML |
| 17-5438-02 | MABSELECT SURE 200 ML |
| 17-5448-01 | Capto Blue 25 ml |
| 17-5474-01 | MabSelect SuRe LX 25 ML |
| 17-5474-02 | MabSelect SuRe LX 200 ML |
| 17-6002-35 | IMMUNOPRECIP STARTER PACK |
| 18-1022-29 | HB AFFINITY CHROMATOGRAPHY |
| 18-1037-46 | ANTIBODY PURIFICATION HB |
| 28-9355-97 | Dextrin Sepharose High Perform |
| 28-9355-98 | Dextrin Sepharose High Perform |
| 28-9356-00 | StrepTactin Sepharose High Per |
| 28-9805-82 | MembraneProteinPurificationKit |
| 17-5468-15 | HiScreen Capto SP ImpRes |
| 17-5470-15 | HiScreen Capto Q ImpRes |
| 17-5474-15 | HiScreen MabSelect SuRe LX |
Glutathione Sepharose™ 4 Fast Flow is used for the purification of GST-tagged proteins from bacterial lysates and other S-tranferases or glutathione-dependent proteins. Mild elution conditions preserve protein antigenicity and function. Sepharose™ 4 Fast Flow provides good flow properties and is excellent for scaling up.
For more information of the full range of products included in the Glutathione S-transferase (GST*) Gene Fusion System, see Introduction to Glutathione S-Transferase (GST) Gene Fusion System. A more comprehensive guide to the GST Gene Fusion System is available in handbook 18-1157-58, see Media Reference Books, Software and Video Cassettes.
Glutathione Sepharose™ 4 Fast Flow 用于从细菌裂解物和其他 S-转移酶或谷胱甘肽依赖性蛋白中纯化 GST 标签蛋白。 温和的洗脱条件保留了蛋白质的抗原性和功能。 Sepharose™ 4 Fast Flow 具有良好的流动特性，非常适合放大。
有关谷胱甘肽 S-转移酶 (GST*) 基因融合系统中包含的全系列产品的更多信息，请参阅谷胱甘肽 S-转移酶 (GST) 基因融合系统简介。 手册 18-1157-58 中提供了更全面的 GST 基因融合系统指南，请参阅媒体参考书、软件和录像带。
| TECHNICAL SPECIFICATIONS | |
| Ligand | Glutathione |
| Ligand coupling method | Epoxy activation |
| Matrix | Highly cross-linked agarose, 4% |
| Average particle size | 90 μm |
| Binding capacity* | » 10 mg recombinant GST/ml medium |
| pH stability | 3-12 |
| Chemical stability | All commonly used aqueous buffers, e.g. 1 M acetate, pH 6.0, 0.1 M NaOH, 70% ethanol, 6 M guanidine hydrochloride (1 h at room temperature) |
| Storage | 20% ethanol |
| Storage temperature | 4°C to 30°C |
| *Binding of GST to glutathione is flow-rate dependent and lower flow rates often increase the binding capacity. This is important to consider during sample loading and elution.GST 与谷胱甘肽的结合取决于流速，通常流速较低 增加结合能力。 在采样过程中考虑这一点很重要 加载和洗脱。 |
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