ProSpec-CD14 Human CHO,CD14 人重组CHO PRO-536

  • CD14 Human, CHO

  • CD14 Human Recombinant, CHO
  • PRO-536

Catalogue number

PRO-536

Synonyms

Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein, CD14.

Introduction

CD14 receptor) is expressed strongly on monocytes and macrophage and weakly on the surface of neutrophils. CD14 is anchored to cells by linkage to glycosylphosphatidylinositol and functions as a high affinity receptor for complexes of LPS and LPS binding protein . Soluble CD14, also binding to LPS, acts at physiological concentration as an LPS agonist and has, at higher concentrations, an LPS antagonizing effect in cell activation. CD14 has been shown to bind apoptotic cells.
CD14 受体)在单核细胞和巨噬细胞上强烈表达,在中性粒细胞表面表达较弱。 CD14 通过与糖基磷脂酰肌醇连接而锚定在细胞上,并作为 LPS 和 LPS 结合蛋白复合物的高亲和力受体发挥作用。 可溶性 CD14 也与 LPS 结合,在生理浓度下作为 LPS 激动剂起作用,并且在较高浓度下,在细胞活化中具有 LPS 拮抗作用。 CD14 已显示与凋亡细胞结合。

Description

The CD14 is produced from human CD14 transfected CHO-cells. Before transfection the complete human CD14-cDNA was amplified by PCR and cloned into expression vector p-POL-DHFR. The myeloid differentiation antigen CD14 acts as the major receptor for bacterial LPS. The dominant form of the recombinant wild type CD14 is the 50-kDa protein containing 335 amino acids and lacks the GPI-Anchor.
CD14 由人 CD14 转染的 CHO 细胞产生。 在转染之前,通过 PCR 扩增完整的人 CD14-cDNA 并克隆到表达载体 p-POL-DHFR 中。 骨髓分化抗原 CD14 是细菌 LPS 的主要受体。 重组野生型 CD14 的主要形式是含有 335 个氨基酸的 50-kDa 蛋白质,并且缺乏 GPI-Anchor。

Source

CHO-cells.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

CD14 was lyophilized from a concentrated protein solution containing phosphate-buffered saline, pH 7.2.

Solubility

It is recommended to reconstitute the lyophilized CD14 in sterile 18MΩ-cm H2O not less than 100µg/ml. Further dilutions should be made with phosphate buffered saline .

Stability

Lyophilized CD14 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CD14 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

Purity

Greater than 95.0% as determined by -PAGE.

Biological Activity

Up to 20 µg/ml CD14 inhibit binding of FITC-LPS to 600,000 CD14+CHO transfectants .

Usage

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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