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NT5E Human
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5′-Nucleotidase Ecto Human Recombinant
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PRO-1853
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Catalogue number
PRO-1853
Synonyms
NT, eN, NT5, NTE, eNT, CD73, E5NT, 5′-nucleotidase, 5′-NT, Ecto-5′-nucleotidase, NT5E.
Introduction
NT5E, also known as CD73, is a plasma membrane protein which catalyzes the conversion of extracellular nucleotides into membrane-permeable nucleosides. NT5E is functions as a determinant of lymphocyte differentiation. Defects in NT5E can cause the calcification of joints and arteries.
NT5E，也称为 CD73，是一种质膜蛋白，可催化细胞外核苷酸转化为可透过膜的核苷。 NT5E 是淋巴细胞分化的决定因素。 NT5E 的缺陷会导致关节和动脉的钙化。
Description
NT5E Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 247amino acids and having a molecular mass of 27kDa. NT5E is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Source
E.coli.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
NT5E protein solution containing 20mM Tris-HCl buffer , 0.15M NaCl, 10% glycerol and 1mM DTT.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .
Avoid multiple freeze-thaw cycles.
For long term storage it is recommended to add a carrier protein .
Avoid multiple freeze-thaw cycles.
如果整个小瓶将在 2-4 周内使用，请在 4°C 下储存。 储存，在 -20°C 下冷冻更长时间。
对于长期储存，建议添加载体蛋白。
避免多次冻融循环。
对于长期储存，建议添加载体蛋白。
避免多次冻融循环。
Purity
Greater than 90% as determined by -PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MWELTILHTN DVHSRLEQTS EDSSKCVNAS RCMGGVARLF TKVQQIRRAE PNVLLLDAGD QYQGTIWFTV YKGAEVAHFM NALRYDAMAL GNHEFDNGVE GLIEPLLKEA KFPILSANIK AKGPLASQIS GLYLPYKVLP VGDEVVGIVG YTSKETPFLS NPGTNLVFED EITALQPEVD KLKTLNVNKI IALGHSGFEM DKLIAQKVRG VDVVVGGHSN TFLYTGN.
Usage
ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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CD73 Antibody
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NT5E Mouse