ProSpec-ENA 78 Human/CHM-331,上皮中性粒细胞激活蛋白 78 人重组/CHM-331

ENA 78 Human

  • Epithelial Neutrophil-Activating Protein 78 Human Recombinant
  • CHM-331

Catalogue number

CHM-331

Synonyms

Small inducible cytokine B5, CXCL5, Epithelial-derived neutrophil-activating protein 78, Neutrophil-activating peptide ENA-78, ENA-78, chemokine ligand 5, SCYB5.

Introduction

趋化因子配体 5 是一种小细胞因子,属于 CXC 趋化因子家族,也称为上皮衍生的中性粒细胞激活肽 78。 它是在用炎性细胞因子 IL-1 或肿瘤坏死因子-α 刺激细胞后产生的。 在嗜酸性粒细胞中也观察到了 CXCL5 的表达,并且可以被 II 型 IFN 抑制。 这种趋化因子刺激嗜中性粒细胞的趋化性,具有血管生成特性。 它通过与细胞表面趋化因子受体 CXCR2 相互作用引发这些效应。 CXCL5 基因在四个外显子上编码,位于人类第 4 号染色体上,与其他几个 CXC 趋化因子基因一起。 CXCL5 与结缔组织重塑有关。

Description

在大肠杆菌中产生的上皮中性粒细胞激活蛋白 78 人重组体是一种单一的、非糖基化的多肽链,含有 74 个氨基酸,分子量为 8020 道尔顿。
CXCL5 通过专有的色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

The CXCL5 was lyophilized from a concentrated solution in water containing no additives.

Solubility

It is recommended to reconstitute the lyophilized ENA-78 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability

Lyophilized ENA78 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CXCL5 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

Purity

Greater than 95.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

Amino acid sequence

The sequence of the first five N-terminal amino acids was determined and was found to be, Ala- Ala -Val-Leu-Arg.

Biological Activity

The biological activity was determined by measuring the dose dependent mobilization of intracellular calcium with human neutrophils. Significant calcium mobilization is observed with 100ng/mL   of recombinant human ENA-78.

Usage

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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