ProSpec-I TAC Mouse,I-TAC 小鼠重组CHM-026

I TAC Mouse
I-TAC Mouse Recombinant
CHM-026
5µg

20µg

1mg

Catalogue number

CHM-026

Synonyms

C-X-C motif chemokine 11, I-TAC, Small-inducible cytokine B11, Cxcl11, Scyb11.

Introduction

Chemokine ligand 11 is a small cytokine belonging to the CXC chemokinen family. I-TAC is highly expressed in peripheral blood leukocytes, pancreas and liver, with moderate levels in thymus, spleen and lung and low expression levels were in small intestine, placenta and prostate. Gene expression of CXCL11 is strongly induced by IFN-g and IFN-b, and weakly induced by IFN-a. The I-TACchemokine elicits its effects on its target cells by interacting with the cell surface chemokine receptor CXCR3, with a higher affinity than do the other ligands for this receptor, CXCL9 and CXCL10. I-TAC is chemotactic for activated T cells.The CXCL11 gene is located on human chromosome 4 along with many other members of the CXC chemokine family.

趋化因子配体 11 是属于 CXC 趋化因子家族的小细胞因子。 I-TAC在外周血白细胞、胰腺和肝脏中高表达，在胸腺、脾脏和肺中表达中等，在小肠、胎盘和前列腺中低表达。 CXCL11的基因表达受IFN-g和IFN-b强烈诱导，而受IFN-a弱诱导。 I-TAC趋化因子通过与细胞表面趋化因子受体 CXCR3 相互作用，引发其对靶细胞的影响，其亲和力高于该受体的其他配体 CXCL9 和 CXCL10。 I-TAC 对活化的 T 细胞具有趋化性。CXCL11 基因与 CXC 趋化因子家族的许多其他成员一起位于人类第 4 号染色体上。

Description

I TAC Mouse Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 79 amino acids and having a molecular mass of 9.1kDa.

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

I TAC protein was lyophilized from a 0.2 µm filtered concentrated solution in 10 mM Sodium Citrate, pH 4.0, with 600 mM NaCl.

Solubility

It is recommended to reconstitute the lyophilized I TAC in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability

Lyophilized I TAC although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution I TAC should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

冻干的 I TAC 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。重组后，I TAC 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Purity

Greater than 98.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

Amino acid sequence

FLMFKQGRCL CIGPGMKAVK MAEIEKASVI YPSNGCDKVE VIVTMKAHKR QRCLDPRSKQ ARLIMQAIEK KNFLRRQNM.

Biological Activity

Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using murine CXCR3 transfected 293 cells is in a concentration of 10-100 ng/ml corresponding to a specific activity of 10,000-100,000 IU/mg.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

I TAC Mouse