SDF-1, CXCL12, Pre-B cell growth-stimulating factor, PBSF, hIRH, chemokine ligand 12, SDF1, SDF1A, TPAR1, SCYB12, SDF-1a, TLSF-a.
SDF-1 is small cytokine belonging to the chemokine family that is officially designated Chemokine ligand 12 . It is produced in two forms, SDF-1?/CXCL12a and SDF-1?/CXCL12b, by alternate splicing of the same gene. Chemokines are characterized by the presence of four conserved cysteines, which form two disulfide bonds. The CXCL12 proteins belong to the group of CXC chemokines, whose initial pair of cysteines are separated by one intervening amino acid. CXCL12 is strongly chemotactic for lymphocytes and has been implicated as an important cell co-ordinator during development. During embryogenesis it directs the migration of hematopoietic cells from foetal liver to bone marrow. Mice which were knocked-out for CXCL12 gene were lethal before the birth or within just 1 hour of life. As another role, CXCL12a alters also the electrophysiology of neurons. CXCL12 was shown to be expressend in many tissues in mice .
The receptor for this chemokine is CXCR4, which was previously called fusin. This CXCL12-CXCR4 interaction used to be considered exclusive , but recently it was suggested that CXCL12 is also bound by CXCR7 receptor.
The gene for CXCL12 is located on human chromosome 10. In human and mouse both CXCL12 and CXCR4 show high identity of sequence: 99% and 90%, respectively.
SDF-1 是属于趋化因子家族的小细胞因子，正式命名为趋化因子配体 12。它通过同一基因的交替剪接以两种形式产生，SDF-1?/CXCL12a 和 SDF-1?/CXCL12b。趋化因子的特征在于存在四个保守的半胱氨酸，它们形成两个二硫键。 CXCL12 蛋白属于 CXC 趋化因子组，其初始半胱氨酸对由一个中间氨基酸隔开。 CXCL12 对淋巴细胞具有很强的趋化性，并且在发育过程中被认为是重要的细胞协调因子。在胚胎发生过程中，它指导造血细胞从胎儿肝脏迁移到骨髓。 CXCL12 基因被敲除的小鼠在出生前或出生后仅 1 小时内都是致命的。作为另一个作用，CXCL12a 也改变了神经元的电生理。 CXCL12 显示在小鼠的许多组织中表达。
这种趋化因子的受体是 CXCR4，以前称为融合蛋白。这种 CXCL12-CXCR4 相互作用曾经被认为是排他性的，但最近有人提出 CXCL12 也与 CXCR7 受体结合。
CXCL12 的基因位于人类第 10 号染色体上。在人类和小鼠中，CXCL12 和 CXCR4 均显示出高度的序列同一性：分别为 99% 和 90%。
Stromal Cell-Derived Factor-1 alpha Human Recombinant produced in E.Coli is a non-glycosylated, Polypeptide chain containing 68 amino acids and having a molecular mass of 8004 Dalton.
The SDF-1a is purified by proprietary chromatographic techniques.
Sterile Filtered White lyophilized powder.
The protein was lyophilized from a concentrated sterile solution containing no additives.
It is recommended to reconstitute the lyophilized Stromal Cell-Derived Factor-1a in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Lyophilized SDF-1a although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CXCL12 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.
Greater than 98.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.
Amino acid sequence
KPVSLSYRCP CRFFESHVAR ANVKHLKILN TPNCALQIVA RLKNNNRQVC IDPKLKWIQE YLEKALNK
The specific activity as determined by its ability to chemoattract human peripheral T cells activated with PHA and IL-2 using a concentation of 20-80 ng/ml corresponding to a Specific Activity of 12,500-50,000IU/mg.
1.Title: Stromal Cell-Derived Factor-1a Promotes Neuroprotection, Angiogenesis, and Mobilization/Homing of Bone Marrow- Derived Cells in Stroke Rats.
Publication: THE JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS Copyright © 2008 by The American Society for Pharmacology and Experimental Therapeutics JPET 324:834–849, 2008. Vol. 324, No. 2 127746/3301181 ed in U.S.A.
Application: Intracerebral administration of SDF-1 resulted in neuroprotection against neurotoxic insult, and it induced increased BM-derived cell targeting to the ischemic brain, thereby reducing the volume of cerebral infarction and improving neural plasticity.
2.Title:MECHANISMS OF BIOMATERIAL MEDIATED FIBROTIC RESPONSES AND STRATEGIES TO
IMPROVE TISSUE REACTIONS TO BIOMATERIAL IMPLANTS.
Publication:THE UNIVERSITY OF TEXAS AT ARLINGTON
Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.