ProSpec,TECK Human,胸腺表达趋化因子人重组体CHM-364

C-C motif chemokine 25, Small-inducible cytokine A25, Thymus-expressed chemokine, Chemokine TECK, CCL25, SCYA25, TECK, Ckb15, MGC150327.

CCL25 is a novel CC chemokine, which is distantly related to other CC chemokines. The mouse CCL25 cDNA has also been cloned and shown to encode a 144 a.a. protein, which exhibits 49% a.a. sequence identity to the human CCL25. Human and mouse CCL25 expression was shown to be greatly restricted to the thymus and small intestine. While dendritic cells are identified as the source of CCL25 production in the thymus, dendritic cells derived from bone marrow do not express CCL25. CCL25 signals through the CCR9 receptor. Teck is possibly involved in T-cell development.
Recombinant human and mouse Teck were shown to be chemotactic for activated macrophages, dendritic cells and thymocytes. The recombinant protein demonstrates chemotactic activity on thymocytes, macrophages, THP-1 cells, and dendritic cells but is inactive on peripheral blood lymphocytes and neutrophils.

CCL25是一种新型CC趋化因子，与其他CC趋化因子关系较远。 小鼠 CCL25 cDNA 也已被克隆并显示编码 144 a.a. 蛋白质，显示 49% a.a. 与人 CCL25 的序列同一性。 人和小鼠的 CCL25 表达被证明极大地局限于胸腺和小肠。 虽然树突细胞被确定为胸腺中 CCL25 产生的来源，但源自骨髓的树突细胞不表达 CCL25。 CCL25 通过 CCR9 受体发出信号。 Teck 可能参与了 T 细胞的发育。
重组人和小鼠 Teck 被证明对活化的巨噬细胞、树突细胞和胸腺细胞具有趋化性。 重组蛋白对胸腺细胞、巨噬细胞、THP-1 细胞和树突状细胞具有趋化活性，但对外周血淋巴细胞和中性粒细胞无活性。

TECK Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 127 amino acids and having a molecular mass of 14.2kDa.
The TECK is purified by proprietary chromatographic techniques.

Escherichia Coli.

Sterile Filtered White lyophilized powder.

Filtered and lyophilized from a concentrated solution in 20mM PB, pH 7.4, 150mM NaCl.

It is recommended to reconstitute the lyophilized TECK in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Lyophilized TECK although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TECK should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

冻干的 TECK 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 重新配制后，TECK 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。
对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Greater than 97.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

QGVFEDCCLA YHYPIGWAVL RRAWTYRIQE VSGSCNLPAA IFYLPKRHRK VCGNPKSREV QRAMKLLDAR NKVFAKLHHN MQTFQAGPHA VKKLSSGNSK LSSSKFSNPI SSSRKNVSLL ISANSGL.

Determined by its ability to chemoattract human monocytes using a concentration range of 1-10ng/ml corresponding to a Specific Activity of 100,000-1,000,000IU/mg.

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.