ProSpec,CCL16 Human,LEC/NCC-4 人重组CHM-237

  • CCL16 Human

  • LEC/NCC-4 Human Recombinant
  • CHM-237

Catalogue number

CHM-237

Synonyms

C-C motif chemokine 16, Small-inducible cytokine A16, IL-10-inducible chemokine, Chemokine LEC, Monotactin-1, Chemokine CC-4, Lymphocyte and monocyte chemoattractant, CCL-16, HCC-4, HCC4, NCC4, NCC-4, Liver Expressed Chemokine, LMC, LCC-1, LCC1, MTN-1, MTN1, SCYL4, ckB12, SCYA16, LEC, ILINCK, MGC117051.

Introduction

Human CCL16, also called HCC-4, liver-expressed chemokine , and lymphocyte and monocyte chemoattractant , is a novel CC chemokine recognized by bioinformatics. NCC-4 cDNA encodes a 120 amino acids along with a 23 amino acids signal peptide that is cleaved to generate 97 amino acid protein. HCC4 is vaguely related to other CC chemokines, showing less than 30% sequence identity. Among CC chemokines, CCL-16 has the largest similarity to HCC-1. 2 potential polyadenylation signals are present on the human HCC-4 gene, and as a result, 2 transcripts containing roughly 1,500 base pairs and 500 base pairs have been detected. HCC-4 is expressed weakly by some lymphocytes, including NK cells, T cells, and some T cell clones. The expression of HCC-4 in monocytes is greatly upregulated in the presence of IL-10.
CCL16 shows chemotactic activity for lymphocytes and monocytes rather than to neutrophils. NCC-4 has potent myelosuppressive activity, suppresses proliferation of myeloid progenitor cells. CCL16 demonstrates chemotactic activity for monocytes and thp-1 monocytes, rather than for resting lymphocytes and neutrophils. HCC-4 induces a calcium flux in thp-1 cells that desensitized prior to the expression of rantes.
人CCL16,又称HCC-4、肝表达趋化因子、淋巴细胞和单核细胞趋化因子,是生物信息学识别的新型CC趋化因子。 NCC-4 cDNA 编码一个 120 个氨基酸以及一个 23 个氨基酸的信号肽,该信号肽被切割产生 97 个氨基酸的蛋白质。 HCC4 与其他 CC 趋化因子模糊相关,显示小于 30% 的序列同一性。在 CC 趋化因子中,CCL-16 与 HCC-1 的相似性最大。人类 HCC-4 基因上存在 2 个潜在的多聚腺苷酸化信号,因此,已检测到包含大约 1,500 个碱基对和 500 个碱基对的 2 个转录本。 HCC-4 被一些淋巴细胞弱表达,包括 NK 细胞、T 细胞和一些 T 细胞克隆。在 IL-10 的存在下,单核细胞中 HCC-4 的表达大大上调。
CCL16 对淋巴细胞和单核细胞显示趋化活性,而不是对中性粒细胞。 NCC-4 具有有效的骨髓抑制活性,抑制骨髓祖细胞的增殖。 CCL16 对单核细胞和 thp-1 单核细胞表现出趋化活性,而不是对静息淋巴细胞和中性粒细胞。 HCC-4 在 thp-1 细胞中诱导钙通量,该细胞在 rantes 表达之前脱敏。

Description

CCL16 Human Recombinant produced in E.Coli is a non-glycosylated, Polypeptide chain containing 97 amino acids and having a molecular mass of 11.2 kDa.
The CCL16 is purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

The CCL16 protein was lyophilized from a concentrated sterile solution containing 20mM PBS pH-7.4 and 0.15M sodium chloride.

Solubility

It is recommended to reconstitute the lyophilized CCL16in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability

Lyophilized CCL16 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CCL16 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.
冻干的 CCL16 虽然在室温下可稳定保存 3 周,但应在 -18°C 以下干燥储存。 复溶后,CCL16 应在 4°C 下储存 2-7 天,以备将来在 -18°C 以下使用。
对于长期储存,建议添加载体蛋白。
请防止冻融循环。

Purity

Greater than 97.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

Amino acid sequence

QPKVPEWVNTPSTCCLKYYEKVLPRRLVVGYRKALNCHLPAIIFVTKRNREVCTNP NDDWVQEYIKDPNLPLLPTRNLSTVKIITAKNGQPQLLNSQ.

Biological Activity

Determined by its ability to chemoattract total human monocytes using a concentration range of 10-100 ng/ml corresponding to a Specific Activity of 10,000-100,000IU/mg.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.