lumiprobe -Universal CPG type I, 500A

Universal CPG type I, 500A

Cat. # Quantity Price Lead time
2646-1g 1 g 39.00$

2646-10g 10 g 351.00$

Universal CPG type I, 500A

Universal CPG type I, 500A

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Items in stock will be shipped on 04.05.2022

Universal CPG type I, 500A

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Universal CPG type I, 500A

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Items in stock will be shipped on 04.05.2022

Universal CPG type I, 500A

Free express shipping from 300$!
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Universal CPG type I, 500A

Free express shipping from 300$!
Your cart total is 0$, and your order qualifies for free express shipping to , ZIP .

The Universal CPG type I, 500A is one of universal supports used to immobilize nucleosides for high-throughput synthesizing oligonucleotides.

The cleavage from the support is followed by deprotection using anhydrous methylamine gas, ammonium hydroxide/methylamine mixture and other basic reagents. Universal support CPG type I is suitable for relatively aggressive environment treatment. Pore size of 500 Å is recommended for the synthesis of oligonucleotides up to 50 bases.

Usage

Coupling: Standard conditions for universal CPG.

Deprotection: 8 hours at 80 °C using ammonium hydroxide or 17 hours at 55 °C; 3 hours at 80 °C using AMA mixture, ammonium hydroxide – 40% methylamine (1:1) or 8 hours at 55 °C using 40% methylamine or 30 minutes at 80 °C; 3 hours at 55 °C; 17 hours at room temperature using 0.4M NaOH in methanol – water (4:1).

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DusQ1 CPG 500

Controlled pore glass (CPG) 500 solid support for synthesis of oligonucleotides with 3’-terminal DusQ1 quencher. Compatible with standard deblocking conditions.

Oligo synthesis details

Pore size, Å: 500
Typical loading, umol/g: 50−95
Coupling conditions: standard coupling, identical to normal nucleobases
Cleavage conditions: AMA mixture, ammonium hydroxide – 40% methylamine (1:1), 3 hours at 80 °C
Deprotection conditions: identical to protected nucleobases

Universal CPG type I, 500A

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