Avitide AVIPure® – AAV9 残留配体检测试剂盒 F970,Cygnus ELISA 试剂盒 F970

The Avitide AVIPure^® – AAV9 Residual Ligand Assay kits are intended for use in quantifying the Avitide AVIPure^® – AAV9 Ligand. This kit (Item Number F970) incorporates a well-qualified sample treatment method to dissociate the ligand from the AAV virus.
AVIPure^® – AAV9 Ligand, immobilized on various chromatography media, is used to purify AAV9 virus. Even when covalently attached, the ligand can leach off of the chromatography support and co-elute with AAV9. Residual AVIPure^® – AAV9 Ligand must be minimized to avoid any adverse patient effects.
The Avitide AVIPure^® – AAV9 Ligand assay is a competition immunoenzymetric assay. The biotinylated ligand is first bound to the streptavidin coated plate. After a wash step to remove any unbound ligand, the antibody, directly labeled with Horse Radish Peroxidase (HRP), as well as the AVIPure^® – AAV9 Ligand is then added to the microtiter plate. After an additional wash step to remove any unbound reactants, the strips are then reacted with tetramethylbenzidine (TMB) substrate. The amount of hydrolyzed substrate is read on a microtiter plate reader and will be directly proportional to the concentration of AVIPure^® – AAV9 Ligand present in the sample. Accurate quantitation is achieved by comparing the signal of unknowns to AVIPure^® – AAV9 Ligand standards assayed at the same time.
This immunoassay provides a simple to use, precise, and highly sensitive method to detect AVIPure^® – AAV9 Ligand to less than 1ng/mL. As such, it can be used as a tool to aid in optimal purification process development and in routine quality control of in-process streams as well as final product. Please use Sample Diluent, Item No. I300, with these kits; also included in the kit.

Avitide AVIPure® – AAV9 Residual Ligand Assay 试剂盒旨在用于定量 Avitide AVIPure® – AAV9 Ligand。该试剂盒（货号 F970）采用了一种合格的样品处理方法，可将配体与 AAV 病毒分离。
AVIPure® – AAV9 配体，固定在各种层析介质上，用于纯化 AAV9 病毒。即使共价连接，配体也可以从色谱支持物上浸出并与 AAV9 共洗脱。残留的 AVIPure® – AAV9 配体必须尽量减少，以避免对患者产生任何不利影响。
Avitide AVIPure® – AAV9 配体测定是一种竞争免疫酶测定。生物素化的配体首先与链霉亲和素涂层板结合。在去除任何未结合配体的洗涤步骤后，将直接用辣根过氧化物酶 (HRP) 标记的抗体以及 AVIPure® – AAV9 配体添加到微量滴定板中。在额外的洗涤步骤以去除任何未结合的反应物后，然后将条与四甲基联苯胺 (TMB) 底物反应。在微量滴定板阅读器上读取水解底物的量，并将与样品中存在的 AVIPure® – AAV9 配体的浓度成正比。通过将未知信号与同时检测的 AVIPure® – AAV9 配体标准品进行比较，实现准确定量。
这种免疫测定法提供了一种简单易用、精确且高度灵敏的方法，可将 AVIPure® – AAV9 配体检测到低于 1ng/mL。因此，它可以作为一种工具来帮助优化纯化工艺开发，以及对过程中物流和最终产品进行常规质量控制。请在这些套件中使用样品稀释剂，货号 I300；也包含在套件中。

请联系 Repligen 咨询 AVIPure® – AAV9 树脂。

F970 PI Avitide AVIPure® – AAV9 ELISA

Sample Treatment Plate

Stop Solution

TBS Wash Concentrate, 20X

TMB Substrate for ELISA

AVIPure^® Sample Diluent

Anti-AVIPure^® – AAV9 Ligand:HRP

Streptavidin coated microtiter strips

AVIPure^® – AAV9 Ligand Standards

Biotinylated AVIPure^® – AAV9 Ligand

STANDARDS SDS

CONJUGATE SDS

TMB SUBSTRATE SDS

WASH CONCENTRATE SDS – F004

STOP SOLUTION SDS – F006

MICROTITER PLATE SDS

DILUENTS SDS

Request a Qualification summary

Request a Certificate of Analysis

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