用于 Gyrolab® 的 HEK 293 HCP 检测试剂套装 G650S,Cygnus ELISA 试剂盒 G650S

HEK 293 HCP Assay Reagent Set for Gyrolab^® is designed for determining the presence of host cell protein contamination in products manufactured by expression in HEK 293 cell lines. This Reagent Set should be used in conjunction with a Gyrolab^® Bioaffy™ 1000 HC Assay Toolbox available from Gyros Protein Technologies. This simple to use, high throughput, and semi-quantitative assay is a method to aid in optimal purification process development, process control, routine quality control and product lot release. It is derived from the same antibodies and antigen used in the Cygnus 3rd Generation HEK 293 HCP ELISA kit, Cat# F650S. The reagents are “generic” in the sense that they are intended to react with essentially all the host cell proteins (HCPs) that could contaminate the product independent of the purification process. The antibodies have been generated in goats and rabbits and antigen affinity purified using HEK 293 HCPs found in protein free conditioned media. The combination of the Reagent Set and Bioaffy 1000 HC Assay Toolbox is optimized for use in Gyrolab automated immunoassay systems enabling generation of 96 data points in 75 minutes without manual intervention. The results are evaluated using Gyrolab Evaluator software.

适用于 Gyrolab® 的 HEK 293 HCP 检测试剂套装旨在确定在 HEK 293 细胞系中表达生产的产品中是否存在宿主细胞蛋白污染。该试剂组应与 Gyros Protein Technologies 提供的 Gyrolab® Bioaffy™ 1000 HC 检测工具箱一起使用。这种简单易用、高通量和半定量的检测方法有助于优化纯化工艺开发、工艺控制、常规质量控制和产品批次放行。它源自 Cygnus 第三代 HEK 293 HCP ELISA 试剂盒 Cat# F650S 中使用的相同抗体和抗原。试剂是“通用的”，因为它们旨在与基本上所有可能污染产品的宿主细胞蛋白 (HCP) 反应，而与纯化过程无关。抗体已在山羊和兔子中产生，抗原亲和性纯化使用HEK 293 HCP 存在于无蛋白质条件培养基中。试剂组和 Bioaffy 1000 HC 检测工具箱的组合经过优化，可用于 Gyrolab 自动免疫检测系统，无需人工干预即可在 75 分钟内生成 96 个数据点。使用 Gyrolab 评估结果评估软件。