J.T.Baker® BAKERBOND® PROchievA™ 蛋白 A Mix-N-Go™ F965,Cygnus ELISA 试剂盒 F965

ELISA Kits

Protein A ELISA Kits

J.T.Baker® BAKERBOND® PROchievA™ Protein A Mix-N-Go™

Description

This kit is designed to quantify the recombinant Protein A ligands used in the Avantor J.T.Baker® BAKERBOND® PROchievA™
chromatographic resins. It features an assay protocol that eliminates sample boiling and the subsequent centrifugation step, thereby reducing assay time by more than 1 hour. This assay provides a simple to use, precise, and highly sensitive method to detect Avantor J.T.Baker® BAKERBOND® PROchievA™ Protein A impurity to less than 300 pg/mL in the presence of up to mg/mL quantities of humanized monoclonal antibodies. As such, this kit can be used as a tool to aid in optimal purification process development and in routine quality control of in-process streams as well as final product. Please use Sample Diluent, Item No. I800, with these kits; also included in the kit.

该试剂盒设计用于定量 Avantor J.T.Baker® BAKERBOND® PROchievA™ 中使用的重组蛋白 A 配体色谱树脂。 它的特点是消除样品煮沸和随后的离心步骤的测定方案,从而将测定时间减少超过 1 小时。 该检测方法提供了一种简单易用、精确且高灵敏度的方法,可在存在高达 mg/mL 量的人源化单克隆抗体的情况下检测低于 300 pg/mL 的 Avantor J.T.Baker® BAKERBOND® PROchievA™ 蛋白 A 杂质。 因此,该套件可用作帮助优化纯化工艺开发以及对过程中物流和最终产品进行常规质量控制的工具。 请在这些试剂盒中使用样品稀释剂,货号 I800; 也包含在套件中。

Product Information

Product Type:
ELISA Kit
Storage:
2°C to 8°C
Assay format:
96 Well Plate
Time to result:
~1 hr. 45 min
LOD:
~100 pg/mL
LLOQ:
700 pg/mL
Recommended Diluent:
I800

Product Insert

F965 PI Avantor Protein A ELISA

Kit Components

Sample Treatment Plate
Stop Solution
TBS Wash Concentrate, 20X
TMB Substrate for ELISA
Mix-N-Go™ Sample Diluent A
Mix-N-Go Denaturing Buffer
Anti-PROchievA™:HRP
Polyclonal Anti-PROchievA™ coated microtiter strips
PROchievA™ Protein A Standards Set, A-H, 1 ml

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