ELISA Kits
Host Cell Protein ELISA Kits
Mammalian HCP ELISA Kits
HEK 293 HCP ELISA Kit, 3G
Description
Important Change Notification Memo: HEK 293 HCP ELISA Kit, 3G Resupply 1
Expression of viral vectors in HEK 293 cells is a widely used procedure for obtaining sufficient quantities of a desired virus. The manufacturing and purification process of these products leaves the potential for impurities by host cell proteins (HCPs) from HEK 293 cells. HCP impurities can result in adverse toxic or immunological reactions and thus it is desirable to reduce these impurities to the lowest levels practical.
The polyclonal antibodies used in this kit have been generated against and affinity-purified using a mild lysate of HEK 293 cells to obtain HCPs typically encountered in initial product recovery steps. This kit can be used as a process development tool to monitor the optimal removal of host cell impurities as well as in routine final product release testing when comprehensively characterized using samples from your process.
The new Resupply 1 antibodies have recently been generated for an ELISA format to match the current F650S kit performance as close as possible. Similar to the anti-HEK 293 HCP antibodies used in the current F650S kit, the new antibodies have broad coverage of ~90% to HEK 293 HCPs as determined by Antibody Affinity Extraction with 2D PAGE/Silver Stain and LC-MS. The HEK 293 HCP ELISA Kit, 3G, Resupply 1, Cat. No. F650S, will replace the current HEK 293 HCP ELISA Kit, 3G, Cat. No F650S. Kits containing the new resupply antibodies will maintain the same catalog number, F650S, and will be identified by the lot numbers starting with characters “R1”. Both kits follow the same assay protocol and have LOQ of 1 ng/mL. Please use Sample Diluent, Cat. No. I700, with these kits.
在 HEK 293 细胞中表达病毒载体是一种广泛使用的方法,用于获得足够数量的所需病毒。这些产品的制造和纯化过程留下了来自 HEK 293 细胞的宿主细胞蛋白 (HCP) 杂质的可能性。 HCP 杂质会导致不利的毒性或免疫反应,因此希望将这些杂质减少到实际的最低水平。
该试剂盒中使用的多克隆抗体已针对 HEK 293 细胞的温和裂解物生成并进行亲和纯化,以获得初始产品回收步骤中通常遇到的 HCP。该试剂盒可用作工艺开发工具,用于监测宿主细胞杂质的最佳去除情况,以及在使用您的工艺样品进行全面表征时的常规最终产品放行测试。
最近为 ELISA 格式生成了新的 Resupply 1 抗体,以尽可能接近当前 F650S 试剂盒的性能。与当前 F650S 试剂盒中使用的抗 HEK 293 HCP 抗体类似,新抗体对 HEK 293 HCP 的广泛覆盖率约为 90%,这是通过使用 2D PAGE/银染色和 LC-MS 进行抗体亲和提取确定的。 HEK 293 HCP ELISA 试剂盒,3G,Resupply 1,Cat.编号 F650S,将取代当前的 HEK 293 HCP ELISA 试剂盒,3G,Cat。没有 F650S。包含新的补给抗体的试剂盒将保持相同的目录号 F650S,并将通过以字符“R1”开头的批号进行识别。两种试剂盒都遵循相同的检测方案,LOQ 为 1 ng/mL。请使用样品稀释剂,猫。编号 I700,带有这些套件。
Product Information
Product Insert
F650S PI HEK 293 ELISA
Kit Components
Product FAQs
- Can the ELISA Protocol in the Product Insert be Modified?
- Calibration of HCP assays
- How do I Maintain Quality Control of ELISA?
- What About Automation of Immunoassays?
- What is the Storage and Stability of ELISA Kits?
- What Issues to Consider When Diluting Samples?
- Why Perform Dual Wavelength Analysis?
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