percoll细胞分离液/100ML/瓶

Percoll is a well-referenced media for density gradient centrifugation of cells, viruses, and subcellular particles. Percoll is composed of colloidal silica coated with polyvinylpyrrolidone (PVP)

Percoll 是一种用于细胞、病毒和亚细胞颗粒的密度梯度离心的广为人知的介质。 Percoll 由涂有聚乙烯吡咯烷酮 (PVP) 的胶体二氧化硅组成

• For separation of cells, subcellular particles and larger viruses (down to ~ 70S) under gentle conditions which preserve viability and morphological integrity.
• Non-toxic to cells.
• Adjustable to physiological ionic strength and pH.
• Gradients can either be preformed or spontaneously generated by centrifugation at moderate speeds in an angle-head rotor.
• Gradients are iso-osmotic throughout and cover a range of densities up to 1.3 g/ml.
• 用于在保持活力和形态完整性的温和条件下分离细胞、亚细胞颗粒和较大的病毒（低至约 70S）。
  对细胞无毒。
  可根据生理离子强度和 pH 值进行调节。
  梯度既可以预先形成，也可以通过在角头转子中以中等速度离心而自发产生。
  梯度在整个过程中都是等渗的，并覆盖了高达 1.3 g/ml 的密度范围。

Percoll is a low viscosity density gradient medium for preparation of cells, subcellular particles, and larger viruses. The low viscosity of the medium enables cell preparation on preformed gradients in only a few minutes using low centrifugal forces (200 to 1000 × g). The medium is avaialble in easy-to-open, resealable 250 ml and 1 l bottles.
Percoll consists of silica particles (15 to 30 nm diameter) coated with non-dialyzable polyvinylpyrrolidone (PVP). Free PVP is present at only 1% to 2%. Percoll is non-toxic, almost chemically inert and does not adhere to membranes. Percoll gradients can be formed within the density range of 1.0 to 1.3 g/ml, and are iso-osmotic throughout.
Percoll can be stored unopened at room temperature for five years. At -20°C, it can only be stored for up to six months. If stored at -20°C, gradients form upon thawing, necessitating a mixing of the bottle before use. Preformed gradients can be stored for weeks without a change in gradient shape, provided that the gradient remains unfrozen. Percoll can be buffered within the pH range 5.5 to 10.0 without any changes in properties. If the pH is dropped below 5.5, gelling may occur. Gelling can also be caused by the presence of divalent cations, an effect which is exacerbated by elevated temperatures. Undiluted Percoll can be resterilized by autoclaving for 30 min at 120°C.

Percoll 是一种低粘度密度梯度培养基，用于制备细胞、亚细胞颗粒和较大的病毒。培养基的低粘度可以在几分钟内使用低离心力（200 至 1000 × g）在预先形成的梯度上制备细胞。该培养基有易于打开、可重新密封的 250 毫升和 1 升瓶装。
Percoll 由涂有不可透析的聚乙烯吡咯烷酮 (PVP) 的二氧化硅颗粒（直径 15 至 30 nm）组成。免费 PVP 仅占 1% 到 2%。 Percoll 是无毒的，几乎是化学惰性的，不会粘附在膜上。 Percoll 梯度可以在 1.0 到 1.3 g/ml 的密度范围内形成，并且始终是等渗的。
Percoll 可以在室温下未开封保存五年。在 -20°C 下，它最多只能储存六个月。如果储存在 -20°C，解冻后会形成梯度，需要在使用前混合瓶子。预先形成的梯度可以存储数周而不改变梯度形状，前提是梯度保持未冻结。 Percoll 可以在 5.5 到 10.0 的 pH 范围内缓冲，而不会改变任何特性。如果 pH 值降至 5.5 以下，可能会发生胶凝。胶凝也可能由二价阳离子的存在引起，升高的温度会加剧这种影响。未稀释的 Percoll 可以通过在 120°C 高压灭菌 30 分钟来重新灭菌。