TNFβ/TNFSF1, human,重组人TNF-β/TNFSF1(肿瘤坏死因子)

重组人TNF-β/TNFSF1(肿瘤坏死因子);TNFβ/TNFSF1
简介:
TNF is secreted by macrophages, monocytes, neutrophils, T-cells, NK-cells following their stimulation by bacterial LPS. Cells expressing CD4 secrete TNF-alpha while CD8 cells secrete little or no TNF-alpha. The synthesis of TNF-alpha is induced by many different stimuli including interferons, IL2, GM-CSF. TNF-β is a potent mediator of inflammatory and immune responses. It belongs to the TNF family of ligands, and signals through TNFR1 and TNFR2. TNF-β is produced by activated T and B lymphocytes, and has similar activities to TNF-α. It mediates a large variety of inflammatory, immunostimulatory, and antiviral responses.
Recombinant Human TNF-β produced in E. coli is is a glycosylated polypeptide chain of 172 amino acids. A fully biologically active molecule,rhTNF-β has a molecular mass of 18.6 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.

TNF 由巨噬细胞、单核细胞、中性粒细胞、T 细胞、NK 细胞在细菌 LPS 刺激后分泌。 表达 CD4 的细胞分泌 TNF-α,而 CD8 细胞分泌很少或不分泌 TNF-α。 TNF-α的合成是由许多不同的刺激诱导的,包括干扰素、IL2、GM-CSF。 TNF-β 是炎症和免疫反应的有效介质。 它属于 TNF 配体家族,通过 TNFR1 和 TNFR2 发出信号。 TNF-β 由活化的 T 和 B 淋巴细胞产生,具有与 TNF-α 相似的活性。 它介导多种炎症、免疫刺激和抗病毒反应。
在大肠杆菌中产生的重组人 TNF-β 是 172 个氨基酸的糖基化多肽链。 rhTNF-β 是一种完全具有生物活性的分子,通过还原 SDS-PAGE 分析其分子量为 18.6 kDa,并在金斯瑞通过色谱技术获得。
别名:Tumor Necrosis Factor-β, human; TNF-beta, human,TNFSF1, Lymphotoxin-alpha (LT-α)
性状描述:
Species:……………………………Human
Source:………………………………E. coli
Measured Molecular Weight:……18.6kDa, observed by reducing SDS-PAGE.
Purity:………………………………> 95% as analyzed by SDS-PAGE.
Formulation:………………………Lyophilized after extensive dialysis against PBS.
Reconstitution:……………………Reconstituted in ddH2O or PBS at 100 μg/ml.
Sequence:

MLPGVGLTPS AAQTARQHPK MHLAHSTLKP AAHLIGDPSK
QNSLLWRANT DRAFLQDGFS LSNNSLLVPT SGIYFVYSQV
VFSGKAYSPK ATSSPLYLAH EVQLFSSQYP FHVPLLSSQK
MVYPGLQEPW LHSMYHGAAF QLTQGDQLST HTDGIPHLVL
SPSTVFFGAF AL
储存条件:Lyophilized recombinant Human Tumor Necrosis Factor-beta (TNF-β) remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, Human TNF-β should be stable up to 1 week at 4°C or up to 3 months at -20°C.For long term storage it is recommended that a carrier protein (example 0.1% BSA) can be added. Avoid repeated freeze-thaw cycles.

运输条件:2~8℃运输
生物活性:
ED50 < 4 pg/mL, measured in a cytotoxicity assay using L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D, corresponding to a specific activity of >2.5 x 108 units/mg.
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用途及描述:科研试剂,广泛应用于分子生物学,药理学等科研方面,严禁用于人体。

【注意】
●我司产品为非无菌包装,若用于细胞培养,请提前做预处理,除去热原细菌,否则会导致染菌。
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英文名字:Tumor Necrosis Factor β(TNF-β),Human,Recombinant
质量标准:>95%,BR