ProSpec-ACRP30 Rat/CYT-831

ACRP30 Rat

Catalogue number

CYT-831

Synonyms

Adipocyte C1q and collagen domain-containing protein, Adipocyte complement-related 30kDa protein, Adipose most abundant gene transcript 1 protein, gelatin-binding protein, Acrp30, AdipoQ, GBP-28, APM-1, ACDC.

脂肪细胞 C1q 和胶原蛋白结构域蛋白、脂肪细胞补体相关 30kDa 蛋白、脂肪最丰富的基因转录物 1 蛋白、明胶结合蛋白、Acrp30、AdipoQ、GBP-28、APM-1、ACDC。

Introduction

Adiponectin, also referred to as Acrp30, AdipoQ and GBP-28, is a recently discovered 244 amino acid protein, the product of the apM1 gene, which is physiologically active and specifically and highly expressed in adipose cells . The protein belongs to the soluble defense collagen super family; it has a collagen-like domain structurally homologous with collagen VIII and X and complement factor C1q-like globular domain. APM-1 forms homotrimers, which are the building blocks for higher order complexes found circulating in serum.

脂联素，也称为Acrp30、AdipoQ和GBP-28，是最近发现的一种244个氨基酸的蛋白质，是apM1基因的产物，具有生理活性，在脂肪细胞中特异性高表达。该蛋白质属于可溶性防御胶原蛋白超家族；它具有与胶原 VIII 和 X 结构同源的胶原样结构域和补体因子 C1q 样球状结构域。 APM-1 形成同源三聚体，它们是血清中循环的高阶复合物的组成部分。

Description

ACRP30 Rat Recombinant produced in 293 cell line is a polypeptide chain containing 238 amino acids and having a total molecular mass of 25.7 kDa. ACRP30 contains a C-Terminal linker and a C-Terminal Flag- tag .

293细胞系产生的ACRP30 Rat Recombinant是一条含有238个氨基酸的多肽链，总分子量为25.7 kDa。 ACRP30 包含一个 C-Terminal 链接器和一个 C-Terminal Flag-tag。

Source

HEK293 cell line.

Physical Appearance

White lyophilized powder.

Formulation

Filtered and lyophilized in 20 mM Tris buffer, 50 mM NaCl, pH 7.5.

Solubility

Add deionized water to a working concentration approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by appropriate sterile filter before using it in the cell culture.

加入去离子水至工作浓度约为 0.5 mg/ml，让冻干颗粒完全溶解。产品不是无菌的！在用于细胞培养之前，请用适当的无菌过滤器过滤产品。

Stability

Lyophilized ACRP30 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution ACRP30 should be stored at 4°C between 2-7 days and for future use below -18°C.

For long term storage it is recommended to add a carrier protein .

Please prevent freeze-thaw cycles.

冻干的 ACRP30 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。复溶后，ACRP30 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。
对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Purity

Greater than 90.0% as determined by -PAGE.

Amino acid sequence

GITATEGPGA LVPPPKETCA GWMAGIPGYP GHNGIPGRDG RDGTPGEKGE KGDAGVLGPK GDPGDAGMTG AEGPRGFPGT PGRKGEPGEA AYMYHSAFSV GLETRVTVPN VPIRFTKIFY NQQNHYDGST GKFHCNIPGL YYFSYHITVY MKDVKVSLFK KDKAVLFTYD QYQEKNVDQA SGSMLLHLEV GDQVWLQVYG EGDNNGLYAD NVNDSTFTGF LLYHDTNAAA DYKDDDDK

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec 的产品仅供实验室研究使用。它们不得用作药物、农业或农药产品、食品添加剂或家用化学品。