CK17/Viability/Cytotoxicity Multiplex Assay Kit-细胞活性/毒性双重检测试剂盒

货号:CK17
细胞活性/毒性双重检测试剂盒
Viability/Cytotoxicity Multiplex Assay Kit
储存条件:0-5度保存
运输条件:室温

特点:

    1. CCK-8与LDH双指标检测毒性,数据更全面。
    2. 准备1次细胞,同时检测2项数据。
    3. 内含500T的CCK-8与LDH检测试剂盒,
    4. 价格优于单独购买。

选择规格:500 tests

关联产品

NO.1. ROS Assay Kit -Highly Sensitive DCFH-DA- ROS检测
NO.2. Annexin V, FITC Apoptosis Detection Kit 细胞凋亡检测
NO.3. Calcein-AM/PI Double Staining Kit 活死细胞双染检测
NO.4. Cell Cycle Assay Kit 细胞周期检测
NO.5. Caspase-3 Assay Kit-Colorimetric- 细胞凋亡检测

试剂盒内含

500 tests Dye Mixture 5mlX1
[500 tests]
.WST-8,1-Methoxy PMS混合液

Cytotoxicity LDH Assay Kit-WST
[500 tests]
.Dye Mixture X1
.Assay Buffer 15.5mlX1
.Lysis Buffer 155mlX1
.Stop Solutin 27.5mlX1

产品概述

细胞毒性检测通常通过检测活细胞数量或者死细胞数量来确定。而为了更加全面的评价细胞毒性情况,多种评价方法相互验证的需求与日俱增。Viability/CytotoxicityMultiplex Assay Kit可以综合评价细胞毒性。试剂盒内含Cell Counting Kit-8 (CCK-8) 和Cytotoxicity LDH Assay Kit-WST® (LDH Assay Kit),分别用于测定活细胞和死细胞数量。

CCK-8通过检测活细胞中脱氢酶活性来确定活细胞数,而LDH Assay Kit通过检测受损细胞膜释放LDH的量来确定死细胞数。LDH Assay Kit可以直接添加到含有细胞的培养基中检测 (一步法)。也可以分离细胞后,加到细胞培养基上清液中检测 (低损伤法,分离的细胞可以做其它实验)。两种细胞毒性评价指标,CCK-8和LDH Assay Kit(低损伤法) 可以使用同一批细胞同时进行实验。

产品特点

不同细胞毒性指标,双重印证。增加结果可信性。

单独检测CCK-8,细胞毒性判断不准确

1、单纯检测活细胞数量(CCK-8、MTT、MTS、XTT等)

2种指标一起检测,增加数据准确性

1、单纯检测活细胞数量(CCK-8、MTT、MTS、XTT等)
2、活细胞检测+死细胞检测(细胞膜损伤)

检测原理

LDH Assay Kit:细胞膜损伤,LDH外漏,通过检测LDH活性判断细胞毒性。

CCK-8:可以使用NADH作为指标来确认活细胞中的代谢活性。

同时检测的操作方法

本试剂盒包括用于测量活细胞的CCK-8和用于测量死细胞的LDH分析试剂盒,两者均可通过平板分析法在相同的吸收波长下进行评估。 此外,同一细胞样本可用于同时评估活细胞和死细胞,从而节省了时间和精力。

性价比高

本产品作为CCK-8(500 tests)与Cytotoxicity LDH Assay Kit(500 tests)组合套装,低于单独购买的总价。适合新接触细胞毒性检测的用户,用于前期摸索实验条件。单独产品链接见下方。大包装购买,单价会降低很多。

Cell Counting Kit-8

Cytotoxicity LDH Assay Kit-WST

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