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• ProteOrange Protein Gel Stain, 5000×

ProteOrange Protein Gel Stain, 5000×

ProteOrange is a protein-binding dye for staining of protein gels, an analog of SYPRO Orange. The fluorophore is a styrene-like zwitterionic dye. It binds proteins selectively in the presence of nucleic acids, polysaccharides, and other molecules. It is also tolerant to SDS, and therefore can be used for SDS-PAGE gel staining. The dye interacts with the SDS coat around proteins in the gel, so it gives more consistent staining between different types of proteins compared to CBB staining, and never exhibits negative staining. It also stains glycoproteins well.

Staining with ProteOrange is about an order of magnitude more sensitive than Coumassie dye staining, and allows to detect about 3 ng of protein per band (30 ng is detection limit for Coumassie).

ProteOrange is supplied as a 5000x solution in DMSO. 1 mL of the solution is enough for the staining of around 200 minigels.

ProteOrange 是一种用于蛋白质凝胶染色的蛋白质结合染料，是 SYPRO Orange 的类似物。 荧光团是一种类似苯乙烯的两性离子染料。 它在存在核酸、多糖和其他分子的情况下选择性地结合蛋白质。 它还耐受 SDS，因此可用于 SDS-PAGE 凝胶染色。 该染料与凝胶中蛋白质周围的 SDS 涂层相互作用，因此与 CBB 染色相比，它可以在不同类型的蛋白质之间提供更一致的染色，并且不会出现负染色。 它还可以很好地染色糖蛋白。

ProteOrange 染色比 Coumassie 染料染色灵敏约一个数量级，每条带可检测约 3 ng 的蛋白质（Coumassie 的检测限为 30 ng）。

ProteOrange 以 DMSO 中的 5000x 溶液形式提供。 1 mL 溶液足以染色约 200 个小凝胶。