H2DCFDA (2′,7′-dichlorodihydrofluorescein diacetate)

  • Products
  • Cell biology
  • Analysis of free radicals
  • H2DCFDA (2′,7′-dichlorodihydrofluorescein diacetate)

H2DCFDA (2′,7′-dichlorodihydrofluorescein diacetate)

标签:FAM (Fluorescein),Microscopy
货号 规格 价格 货期
2247-10mg 10 mg 10 days
2247-100mg 100 mg 110.00$ in stock
2247-250mg 250 mg 190.00$ in stock

H2DCFDA (2′,7′-dichlorodihydrofluorescein diacetate) is a common reagent used for investigating the production of reactive oxygen species in living cells.

H2DCFDA is a non-fluorescent fluorescein derivative (its reduced acetylated form). The reagent begins to emit fluorescence only after cleavage of acetyl groups and the reagent’s oxidation in the cell while converting to 2’,7’-dichlorofluorescein. This is a bright green-fluorescent dye (absorption maximum 511 nm, fluorescence maximum 533 nm). This reagent can be used for assays in living cells and is not compatible with sample fixation.

Acetyl groups in H2DCFDA increase its lipophilicity and improve its cell membrane permeability. Once got into the cell, the dye is deacetylated by cell esterases, thus becoming charged and better fixed inside the cell. Oxidation with reactive oxygen forms results in the formation of a fluorescent product (2’,7’-dichlorofluorescein) and can be detected using various methods, for example with a flow cytometer, plate reader, or fluorescent microscope.


H2DCFDA 是一种非荧光荧光素衍生物(其还原乙酰化形式)。该试剂仅在乙酰基裂解和试剂在细胞中氧化并转化为 2′,7′-二氯荧光素后才开始发射荧光。这是一种亮绿色荧光染料(最大吸收 511 nm,最大荧光 533 nm)。该试剂可用于活细胞检测,与样品固定不兼容。

H2DCFDA 中的乙酰基会增加其亲脂性并改善其细胞膜通透性。一旦进入细胞,染料就会被细胞酯酶脱乙酰化,从而带电并更好地固定在细胞内。用活性氧形式氧化会形成荧光产物(2′,7′-二氯荧光素),可以使用各种方法检测,例如使用流式细胞仪、读板器或荧光显微镜。

Recommendations for using the reagent使用试剂的建议::

  • Use a freshly prepared solution of the reagent (the working solution is not intended for long-term storage because of gradual reagent oxidation).
  • Select an optimal working concentration of the reagent and incubation time required for reagent deacetylation and oxidation for the specific cell line and assay conditions. If there are no protocols recommended for the specific cell line, start with a concentration from 1 to 10 µM and incubation for 30 min.
  • Do not incubate the dye with the cells in the presence of serum because it contains enzymes that cleave H2DCFDA.
  • 使用新配制的试剂溶液(工作溶液不适合长期储存,因为试剂会逐渐氧化)。
    针对特定细胞系和测定条件,选择试剂脱乙酰和氧化所需的最佳试剂工作浓度和孵育时间。 如果没有针对特定细胞系推荐的方案,请从 1 到 10 µM 的浓度开始并孵育 30 分钟。
    不要在存在血清的情况下将染料与细胞一起孵育,因为它含有切割 H2DCFDA 的酶。

H2DCFDA (2′,7′-dichlorodihydrofluorescein diacetate) H2DCFDA (2′,7′-dichlorodihydrofluorescein diacetate) H2DCFDA (2′,7′-dichlorodihydrofluorescein diacetate) H2DCFDA (2′,7′-dichlorodihydrofluorescein diacetate)

H2DCFDA absorbance and emission spectra

H2DCFDA (2′,7′-dichlorodihydrofluorescein diacetate)

H2DCFDA (2′,7′-dichlorodihydrofluorescein diacetate)相关产品

Cyanine5 NHS ester

Amine-reactive Cyanine5 activated ester for the labeling of proteins, peptides, and other molecules.

胺反应性 Cy5 活化酯,用于标记蛋白质、肽和其他分子。

Cyanine5 DBCO

Cyanine5 dye with dibenzocyclooctyne (DBCO) functional group for copper free Click chemistry.

用于无铜点击化学的具有二苯并环辛炔 (DBCO) 官能团的 Cy5 染料。

Cyanine7.5 NHS ester

Amine reactive NHS ester of NIR fluorescent dye Cyanine7.5.


General properties

Appearance: colorless solid
Molecular weight: 487.29
CAS number: 4091-99-0
Molecular formula: C24H16Cl2O7
IUPAC name: 2-(3,6-diacetyloxy-2,7-dichloro-9H-xanthen-9-yl)benzoic acid
Solubility: good in DMSO, ethanol, and DMF
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: 12 months from delivery at −20°C protected from light. Transportation: Up to 3 weeks at room temperature. Protect from moisture. Avoid storage unprotected from light and unnecessary freeze-thaw cycles of the stock solution. If the reagent is used several times, it is strongly recommended to store it in an atmosphere of dry argon or nitrogen.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 511
ε, L⋅mol−1⋅cm−1: 118626
Emission maximum, nm: 533
CF260: 0.17
CF280: 0.14