DusQ2 CPG 1000

  • Products
  • DNA
  • CPG solid supports
  • DusQ2 CPG 1000

DusQ2 CPG 1000

标签:CPG (controlled pore glass) solid supports for oligonucleotide synthesis
货号 规格 价格 货期
15960 100 mg 10 days
35960 500 mg 530.00$ in stock
45960 1 g 990.00$ in stock
55960 5 g please inquire in stock

This modified support is intended for the synthesis of oligonucleotides with DusQ2 quencher at the 3’ end. Pore size of 1000 Å is recommended for the synthesis of oligonucleotides of up to 120 bases in length.

DusQ2 is a fluorescence quencher with absorption within the range of 560 to 670 nm. It is ideal for effective FRET quenching of fluorophores with emission in this range. The quencher is also used in hybridization probes with static and combined quenching. Its quenching effectiveness does not depend very much on overlapping of fluorophore and quencher spectra, thus allowing for effective quenching of the broad spectrum of fluorophores, including those with emission in the red and far-red part of the spectrum. Thus, DusQ2 can be used with such fluorophores (including but not limited to) as Cyanine3, TAMRA, ROX, Cyanine3.5, Quasar® 570, Pulsar® 650, Cyanine5, Quasar 670, Cyanine5.5, and Quasar 705.

这种修饰的支持物旨在合成 3′ 端具有 DusQ2 淬灭剂的寡核苷酸。 1000 Å 的孔径被推荐用于合成长度不超过 120 个碱基的寡核苷酸。

DusQ2 是一种荧光猝灭剂,吸收范围为 560 至 670 nm。 它非常适合在此范围内发射荧光团的有效 FRET 猝灭。 猝灭剂还用于具有静态和组合猝灭的杂交探针。 它的猝灭效果不太依赖于荧光团和猝灭剂光谱的重叠,因此可以有效猝灭广谱荧光团,包括在光谱的红色和远红色部分发射的荧光团。 因此,DusQ2 可以与诸如 Cy3、TAMRA、ROX、Cy3.5、Quasar® 570、Pulsar® 650、Cy5、Quasar 670、Cy5.5 和 Quasar 705 等荧光团(包括但不限于)一起使用。

Usage 用法

Coupling: Standard conditions identical to normal nucleobases.

Deprotection: 2 hours at room temperature using concentrated ammonia or 10 min at 65 °C using AMA mixture, concentrated aqueous ammonia/40% methylamine (1:1). Deprotection conditions depend on oligonucleotide composition and nucleobase protecting groups, as well as additional modifications, if present.


脱保护:在室温下使用浓氨水 2 小时或在 65°C 下使用 AMA 混合物、浓氨水/40% 甲胺 (1:1) 脱保护 10 分钟。 脱保护条件取决于寡核苷酸组成和核碱基保护基团,以及其他修饰(如果存在)。

DusQ2 CPG 1000

DusQ2 CPG 1000 DusQ2 CPG 1000

Absorption spectrum of DusQ2

DusQ2 CPG 1000

DusQ2 CPG 1000相关产品

DusQ2 phosphoramidite

DusQ2 is a black quencher dye phosphoramidite for the synthesis of dual labeled probes. Useful for HEX, JOE, ROX, Cyanine5, and other dyes.

DusQ2 是一种黑色猝灭染料亚磷酰胺,用于合成双标记探针。 适用于 HEX、JOE、ROX、Cyanine5 和其他染料。

DusQ2 CPG 500

Controlled pore glass (CPG) 500 modified support for the synthesis of oligonucleotides with DusQ2 quencher at the 3’ end. Compatible with standard deblocking conditions.

受控孔玻璃 (CPG) 500 改进的支持合成寡核苷酸,在 3′ 端具有 DusQ2 淬灭剂。 与标准解块条件兼容。

DusQ1 CPG 1000

Controlled pore glass (CPG) 1000 solid support for synthesis of oligonucleotides with 3’-terminal DusQ1 quencher. Compatible with standard deblocking conditions.

可控孔玻璃 (CPG) 1000 固体支持物,用于合成具有 3′-末端 DusQ1 猝灭剂的寡核苷酸。 与标准解块条件兼容。

General properties

Appearance: dark blue beads
Quality control: NMR 1H and HPLC-MS (95%) of bound reagent, loading measurement, functional testing in oligo synthesis.
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

储存:收到后 24 个月,在 -20°C 避光保存。 运输:在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。

MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 552
ε, L⋅mol−1⋅cm−1: 34000
CF260: 0.31
CF280: 0.26

Oligo synthesis details

Pore size, Å: 1000
Typical loading, umol/g: 70−80