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- DusQ1 CPG 500
DusQ1 CPG 500
货号 | 规格 | 价格 | 货期 |
---|---|---|---|
11960 | 100 mg | – | in stock |
31960 | 500 mg | 530.00$ | in stock |
41960 | 1 g | 990.00$ | in stock |
51960 | 5 g | please inquire | in stock |
This modified support with a pore size of 500 Å is intended for the synthesis of oligonucleotides of up to 50 bases in length modified with non-fluorescent DusQ1 quencher at the 3’ end.
DusQ1 dark quencher exhibits the strongest absorption within the range of 480 to 580 nm; its absorption maximum is at 534 nm. It can be used for combined quenching (a combination of static and dynamic quenching) of many fluorophores, including Biosearch Blue™, Marina Blue™, Edans, Bothell Blue, FAM™, JOE™, VIC™, R6G, HEX™, TET™, CAL Fluor™ Gold 540, and Yakima Yellow™. It can be used for the synthesis of hybridization probes such as TaqMan, Molecular Beacon, Scorpion.
这种孔径为 500 Å 的修饰载体用于合成长度不超过 50 个碱基的寡核苷酸,在 3′ 端用非荧光 DusQ1 猝灭剂修饰。
DusQ1 暗猝灭剂在 480 至 580 nm 范围内表现出最强的吸收; 其吸收最大值为 534 nm。 它可用于多种荧光团的组合猝灭(静态和动态猝灭的组合),包括 Biosearch Blue™、Marina Blue™、Edans、Bothell Blue、FAM™、JOE™、VIC™、R6G、HEX™、TET™ 、CAL Fluor™ Gold 540 和 Yakima Yellow™。 可用于合成 TaqMan、Molecular Beacon、Scorpion 等杂交探针。
Usage 用法
Coupling: Standard conditions identical to normal nucleobases.
Deprotection: 2 hours at room temperature using concentrated ammonia or 10 min at 65 °C using AMA mixture, concentrated aqueous ammonia/40% methylamine (1:1). Deprotection conditions depend on oligonucleotide composition and nucleobase protecting groups, as well as additional modifications, if present.
偶联:与正常核碱基相同的标准条件。
脱保护:在室温下使用浓氨水 2 小时或在 65°C 下使用 AMA 混合物、浓氨水/40% 甲胺 (1:1) 脱保护 10 分钟。 脱保护条件取决于寡核苷酸组成和核碱基保护基团,以及其他修饰(如果存在)。
Absorption spectrum of DusQ1
DusQ1 CPG 500相关产品
HEX phosphoramidite, 6-isomer
HEX phosphoramidite for synthesis of 5’-labeled oligonucleotides.
用于合成 5′ 标记寡核苷酸的 HEX 亚磷酰胺。
Cyanine2 NHS ester minimal dye
Cyanine2 NHS ester minimal dye for protein labeling
用于蛋白质标记的 Cy2 NHS 酯最小染料
BDP TR azide
BDP TR is a red emitting fluorophore for ROX channel. This is an azide for copper catalyzed and copper free Click chemistry conjugation.
BDP TR 是一种用于 ROX 通道的红色发射荧光团。 这是一种用于铜催化和无铜点击化学偶联的叠氮化物。
General properties
Appearance: | purple beads |
Quality control: | NMR 1H and HPLC-MS (95%) of bound reagent, loading measurement, functional testing in oligo synthesis. |
Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
MSDS: | Download |
Product specifications |
Spectral properties
Excitation/absorption maximum, nm: | 522 |
ε, L⋅mol−1⋅cm−1: | 35935 |
CF260: | 0.17 |
CF280: | 0.10 |
Oligo synthesis details
Pore size, Å: | 500 |
Typical loading, umol/g: | 70−80 |