DusQ1 CPG 500

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DusQ1 CPG 500

标签:CPG (controlled pore glass) solid supports for oligonucleotide synthesis
货号 规格 价格 货期
11960 100 mg in stock
31960 500 mg 530.00$ in stock
41960 1 g 990.00$ in stock
51960 5 g please inquire in stock

This modified support with a pore size of 500 Å is intended for the synthesis of oligonucleotides of up to 50 bases in length modified with non-fluorescent DusQ1 quencher at the 3’ end.

DusQ1 dark quencher exhibits the strongest absorption within the range of 480 to 580 nm; its absorption maximum is at 534 nm. It can be used for combined quenching (a combination of static and dynamic quenching) of many fluorophores, including Biosearch Blue™, Marina Blue™, Edans, Bothell Blue, FAM™, JOE™, VIC™, R6G, HEX™, TET™, CAL Fluor™ Gold 540, and Yakima Yellow™. It can be used for the synthesis of hybridization probes such as TaqMan, Molecular Beacon, Scorpion.

这种孔径为 500 Å 的修饰载体用于合成长度不超过 50 个碱基的寡核苷酸,在 3′ 端用非荧光 DusQ1 猝灭剂修饰。

DusQ1 暗猝灭剂在 480 至 580 nm 范围内表现出最强的吸收; 其吸收最大值为 534 nm。 它可用于多种荧光团的组合猝灭(静态和动态猝灭的组合),包括 Biosearch Blue™、Marina Blue™、Edans、Bothell Blue、FAM™、JOE™、VIC™、R6G、HEX™、TET™ 、CAL Fluor™ Gold 540 和 Yakima Yellow™。 可用于合成 TaqMan、Molecular Beacon、Scorpion 等杂交探针。

Usage 用法

Coupling: Standard conditions identical to normal nucleobases.

Deprotection: 2 hours at room temperature using concentrated ammonia or 10 min at 65 °C using AMA mixture, concentrated aqueous ammonia/40% methylamine (1:1). Deprotection conditions depend on oligonucleotide composition and nucleobase protecting groups, as well as additional modifications, if present.


脱保护:在室温下使用浓氨水 2 小时或在 65°C 下使用 AMA 混合物、浓氨水/40% 甲胺 (1:1) 脱保护 10 分钟。 脱保护条件取决于寡核苷酸组成和核碱基保护基团,以及其他修饰(如果存在)。

DusQ1 CPG 500 DusQ1 CPG 500 DusQ1 CPG 500

Absorption spectrum of DusQ1

DusQ1 CPG 500

DusQ1 CPG 500相关产品

HEX phosphoramidite, 6-isomer

HEX phosphoramidite for synthesis of 5’-labeled oligonucleotides.

用于合成 5′ 标记寡核苷酸的 HEX 亚磷酰胺。

Cyanine2 NHS ester minimal dye

Cyanine2 NHS ester minimal dye for protein labeling

用于蛋白质标记的 Cy2 NHS 酯最小染料

BDP TR azide

BDP TR is a red emitting fluorophore for ROX channel. This is an azide for copper catalyzed and copper free Click chemistry conjugation.

BDP TR 是一种用于 ROX 通道的红色发射荧光团。 这是一种用于铜催化和无铜点击化学偶联的叠氮化物。

General properties

Appearance: purple beads
Quality control: NMR 1H and HPLC-MS (95%) of bound reagent, loading measurement, functional testing in oligo synthesis.
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 522
ε, L⋅mol−1⋅cm−1: 35935
CF260: 0.17
CF280: 0.10

Oligo synthesis details

Pore size, Å: 500
Typical loading, umol/g: 70−80