DusQ1 CPG 1000

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DusQ1 CPG 1000

CPG (controlled pore glass) solid supports for oligonucleotide synthesis
货号 规格 价格 货期
14960 100 mg 10 days
34960 500 mg 530.00$ in stock
44960 1 g 990.00$ in stock
54960 5 g please inquire in stock

This support with a pore size of 1000 Å is intended for the automated synthesis of oligonucleotides of up to 120 bases in length modified with DusQ1 dark quencher at the 3’ end.

Non-fluorescent DusQ1 quencher exhibits the strongest absorption within the range of 480 to 580 nm; its absorption maximum is at 534 nm. It is suitable for quenching (a combination of static and dynamic quenching) of many fluorophores, including Biosearch Blue™, Marina Blue™, Edans, Bothell Blue, FAM™, JOE™, VIC™, R6G, HEX™, TET™ and Yakima Yellow™. It can be used for the synthesis of hybridization probes such as TaqMan, Molecular Beacon, Scorpion.

这种孔径为 1000 Å 的载体用于自动合成长度达 120 个碱基的寡核苷酸,在 3′ 端用 DusQ1 暗猝灭剂修饰。

非荧光 DusQ1 猝灭剂在 480 至 580 nm 范围内表现出最强的吸收; 其吸收最大值为 534 nm。 它适用于多种荧光团的猝灭(静态和动态猝灭的组合),包括 Biosearch Blue™、Marina Blue™、Edans、Bothell Blue、FAM™、JOE™、VIC™、R6G、HEX™、TET™ 和 Yakima 黄色™。 可用于合成 TaqMan、Molecular Beacon、Scorpion 等杂交探针。

Usage 用法

Coupling: Standard conditions identical to normal nucleobases.

Deprotection: 2 hours at room temperature using concentrated ammonia or 10 min at 65 °C using AMA mixture, concentrated aqueous ammonia/40% methylamine (1:1). Deprotection conditions depend on oligonucleotide composition and nucleobase protecting groups, as well as additional modifications, if present.


脱保护:在室温下使用浓氨水 2 小时或在 65°C 下使用 AMA 混合物、浓氨水/40% 甲胺 (1:1) 脱保护 10 分钟。 脱保护条件取决于寡核苷酸组成和核碱基保护基团,以及其他修饰(如果存在)。

DusQ1 CPG 1000 DusQ1 CPG 1000 DusQ1 CPG 1000

Absorption spectrum of DusQ1

DusQ1 CPG 1000

DusQ1 CPG 1000相关产品

DusQ1 amidite, 5′-terminal

DusQ2 CPG 1000

Controlled pore glass (CPG) 1000 modified support for the synthesis of oligonucleotides with DusQ2 quencher at the 3’ end. Compatible with standard deblocking conditions.

可控孔玻璃 (CPG) 1000 改良后的 3′ 末端带有 DusQ2 淬灭剂的寡核苷酸合成支持。 与标准解块条件兼容。

DusQ1 CPG 500

Controlled pore glass (CPG) 500 solid support for synthesis of oligonucleotides with 3’-terminal DusQ1 quencher. Compatible with standard deblocking conditions.

可控孔玻璃 (CPG) 500 固体支持物,用于合成具有 3′-末端 DusQ1 淬灭剂的寡核苷酸。 与标准解块条件兼容。

General properties

Appearance: red beads
Quality control: NMR 1H and HPLC-MS (95%) of bound reagent, loading measurement, functional testing in oligo synthesis.
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

储存:收到后 24 个月,在 -20°C 避光保存。 运输:在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。

MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 522
ε, L⋅mol−1⋅cm−1: 35935
CF260: 0.17
CF280: 0.10

Oligo synthesis details

Pore size, Å: 1000
Typical loading, umol/g: 70−80